Quick-stop mutant

In the following paragraph, many strands are used, how did they use each strands for the preparation of mutant TCR library? Construction of 2C CDR3α DNA library 2Cα and 2Cβ cDNAs, were cloned into the murine stem cell virus (MSCV) retroviral vector in a bicistronic configuration ...

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so a recessive allele is basicly a mutant gene? ah... well I guess that is beyond the level of my understanding, but thanks for the quick replays.

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So, for a quick answer ... there is no "strand" that tells a cell to die. (Remeber there ... cells to breathe-with and keep you alive. ... and then you die. So esentially the mutant (cancerous) gene doesn't exactly 'tell' the cell/organism when to exactly to die ...

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... as a germline approach (in humans.. due to ethics!). To give you an quick explanation of what would have to happen (for alzheimers disease) i'll ... not a carrier of a common gene; Jen, is a carrier of autosomal dominant mutant APP (amyloid precursor) gene. And because she has 1 copy of the gene.. ...

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