Dictionary » Q » Quenching

Quenching

quenching

1. The process of extinguishing, removing, or diminishing a physical property such as heat or light; e.g., the cooling of a hot metal rapidly by plunging it into water or oil.

2. In beta liquid scintillation counting, the shifting of the energy spectrum from a true to a lower energy; it is caused by a variety of interfering materials in the counting solution, including foreign chemicals and colouring agents.

3. The process of stopping a chemical or enzymatic reaction.

Origin: M. E. Quenchen, fr. O.E. Acwencan

fluorescence quenching, a technique used in investigations dealing with binding of antigens (haptens) by purified antibodies, applicable in cases in which the bound antigen (hapten) absorbs (quenches) light emitted during fluorescence of protein (antibody) excited by ultraviolet light.


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qPCR DNA concentration problem...

Contaminants in your DNA prep that change your quantification (fluorescence quenching or something like that)?

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by canalon
Thu Jan 20, 2011 4:16 pm
 
Forum: Molecular Biology
Topic: qPCR DNA concentration problem...
Replies: 1
Views: 1052

QQ vs anti-QS

Is there any difference between Quorum quenching agent and Anti-Quorum sensing agent?

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by magicsiew
Wed Jul 14, 2010 9:02 am
 
Forum: General Discussion
Topic: QQ vs anti-QS
Replies: 0
Views: 659

SYBR gold in acrylamide gel

... so I don't know if that sort of thing happens or not. Another possibilty, I suppose, is that your sample got stuck in the well, but if there is no quenching, I would guess you would have seen your wells light up. I assume you've done it more than once, so there is no possibility that it's just ...

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by blcr11
Thu Oct 30, 2008 1:53 pm
 
Forum: Molecular Biology
Topic: SYBR gold in acrylamide gel
Replies: 4
Views: 5380

How can I determine lysozyme activity´╝č

... on Micrococcus lysodeikticus cell walls, which are labeled to such a degree that the fluorescence is quenched. Lysozyme action can relieve this quenching, yielding a dramatic increase in fluorescence that is proportional to lysozyme activity. The fluorescence increase can be measured using any ...

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by angelsmile
Tue Oct 21, 2008 2:30 am
 
Forum: Microbiology
Topic: How can I determine lysozyme activity´╝č
Replies: 0
Views: 3386

Re: enriching for DNA palindromes?

This may or may not work, but you could try quenching your annealing reaction by diluting and passing the mix over hydroxyapatite. Maybe you need to do the S1 reaction in the diluted sample prior to passing it over hydroxyapatite; not sure how ...

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by blcr11
Wed Aug 27, 2008 4:25 pm
 
Forum: Molecular Biology
Topic: enriching for DNA palindromes?
Replies: 2
Views: 1888
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