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Quenches

quench

1. To extinguish; to overwhelm; to make an end of; said of flame and fire, of things burning, and figuratively of sensations and emotions; as, to quench flame; to quench a candle; to quench thirst, love, hate, etc. Ere our blood shall quench that fire. (Shak) The supposition of the lady's death Will quench the wonder of her infamy. (Shak)

2. To cool suddenly, as heated steel, in tempering.

Synonym: To extinguish, still, stifle, allay, cool, check.

Origin: OE. Quenchen, AS. Cwencan in acwencan, to extinguish utterly, causative of cwincan, acwincan, to decrease, disappear; cf. AS. Cwinan, acwinan, to waste or dwindle away.


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SYBR gold in acrylamide gel

You have anything in your gel or buffer system that quenches fluorescence? Ethidium bromide, maybe? I don't run SYBR gels, so I don't know if that sort of thing happens or not. Another possibilty, I suppose, is that your sample got stuck in the well, ...

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by blcr11
Thu Oct 30, 2008 1:53 pm
 
Forum: Molecular Biology
Topic: SYBR gold in acrylamide gel
Replies: 4
Views: 5394


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