1. To extinguish; to overwhelm; to make an end of; said of flame and fire, of things burning, and figuratively of sensations and emotions; as, to quench flame; to quench a candle; to quench thirst, love, hate, etc. Ere our blood shall quench that fire. (Shak) The supposition of the lady's death Will quench the wonder of her infamy. (Shak)
Origin: OE. Quenchen, AS. Cwencan in acwencan, to extinguish utterly, causative of cwincan, acwincan, to decrease, disappear; cf. AS. Cwinan, acwinan, to waste or dwindle away.
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You have anything in your gel or buffer system that quenches fluorescence? Ethidium bromide, maybe? I don't run SYBR gels, so I don't know if that sort of thing happens or not. Another possibilty, I suppose, is that your sample got stuck in the well, ...
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