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Quench

quench

1. To extinguish; to overwhelm; to make an end of; said of flame and fire, of things burning, and figuratively of sensations and emotions; as, to quench flame; to quench a candle; to quench thirst, love, hate, etc. Ere our blood shall quench that fire. (Shak) The supposition of the lady's death Will quench the wonder of her infamy. (Shak)

2. To cool suddenly, as heated steel, in tempering.

Synonym: To extinguish, still, stifle, allay, cool, check.

Origin: OE. Quenchen, AS. Cwencan in acwencan, to extinguish utterly, causative of cwincan, acwincan, to decrease, disappear; cf. AS. Cwinan, acwinan, to waste or dwindle away.


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Diabetes

... along with fluids drawn from your tissues. This triggers more frequent urination, which may leave you dehydrated. As you drink more fluids to quench your thirst, you'll urinate even more. 2-Fatigue You may feel fatigued. Many factors can contribute to this. They include dehydration from increased ...

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by einfopedia
Tue Apr 03, 2012 8:31 am
 
Forum: Human Biology
Topic: Diabetes
Replies: 5
Views: 3695

question about LCM

... of interest can help visualize the cells, I don’t have to perform a staining step. Also, I didn’t do the dehydration step since the alcohol could quench the GFP signal remarkably. Then it was difficult for us to capture a single cell using IR laser on LCM machine, what we can do was just to use ...

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by johnmay
Mon Oct 17, 2011 4:43 pm
 
Forum: Molecular Biology
Topic: question about LCM
Replies: 0
Views: 1149

Paraormaldehyde action in immunofluorescence.

... bring it up to 10 mL by adding 1 mL 10x PBS). Does the temperature of the PFA solution affect anything about the fixation? After PFA fixation, I quench PFA with 25 mM glycin for 20 min. What is this last step doing exactly?

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by kk
Fri Oct 16, 2009 12:25 pm
 
Forum: Cell Biology
Topic: Paraormaldehyde action in immunofluorescence.
Replies: 8
Views: 9359

Re: Dose response curves

... whichever is easier. This will be the initial velocity of the reaction in micrmole/min or some such unit. I’m assuming you have a way to either quench the reaction after 1 min, or you’re continuously monitoring the absorbance or fluorescence over time, and you can just “take” the value at 1 ...

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by blcr11
Thu Aug 28, 2008 4:50 pm
 
Forum: Molecular Biology
Topic: Dose response curves
Replies: 1
Views: 2307

1 MOLAR histidine?

... method for polyamine detection using 4-fluoro-3-nitrobenzotrifluoride (FNBT) (Spragg and Hutchings, 1983). The published protocol tells you to quench the reaction with 1 M histidine in 1 M sodium bicarbonate. At first I thought this must be a typo because it's so far above the nominal solubility ...

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by mediocritus
Sun Apr 20, 2008 10:37 pm
 
Forum: Molecular Biology
Topic: 1 MOLAR histidine?
Replies: 6
Views: 4208
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