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Quantify

quantify

To express as a numerical amount.


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FISH and Taqman probes

I want to quantify bacteria , and this bacteria has been detected by using FISH probe(targets 16S). Can I use this FISH probe as my TaqMan probe and design the forward and reverse primers on the two sides of it?

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by 2444256703
Tue Mar 03, 2015 6:28 am
 
Forum: General Discussion
Topic: FISH and Taqman probes
Replies: 0
Views: 110

Taqman probe design using FISH probe (need help)

... will be hydrolysed by Taq DNA polymerase at every replication, thus releasing the fluorescence (quenched in intact probe) that will be measured to quantify the starting level of template. Therefore, you can design primers and probes so that they will fall within the sequence recognized by the FISH ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 543

Taqman probe design using FISH probe (need help)

I’m a newcomer in this forum and in the world of qPCR and I need your help please. I want to quantify bacteria by using RT-PCR (Taqman), this bacteria has been detected (in previous study) by using FISH probe (targets 16S). Can I use this FISH probe (from the paper) as ...

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by biomart
Wed Feb 04, 2015 7:10 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 543

Basic Knowledge about DNA Extraction

... specific bacterial groups and Terminal Restriction Fragment Length Polymorphism (T-RFLP), the technique used to identify, characterize and quantify spatial and temporal patterns in marine bacterioplankton communities and also Sequencing. The basic step of DNA Extraction is lysis of the ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
 
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 752

Re: How can i asses the parasie loading of littorina littoria?

... cercariae when transferred to a vial of water. We could then see the released cercariae when the vial was held up to a light. We never tried to quantify them, but it was a good fast and easy way to find the cercariae we were looking for. The water did not have to contain anything special - just ...

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by marineman
Sun Feb 24, 2013 1:36 pm
 
Forum: Zoology Discussion
Topic: How can i asses the parasie loading of littorina littoria?
Replies: 7
Views: 4651
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