Q bands

... a gDNA of the gene. I used dreamtaq kit and its protocol but unfortunately the expected product (2054 bp) was not there and what I got is several bands below and above my expected product size. Then I have used Phusion hihgfidelity kit but the problem is still there no expected product was there. ...

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Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the ...

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... trying to determine the genotype of some cell samples using PCR and agaorse gel electrophoresis. On one of the samples, I was supposed to find two bands representing a heterozygote for a particular mutated receptor but I could only see one band representing the wild type homozygote. When other ...

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... fractions. after transfering the protein in nitrocellulose membrane when i'm staining the same with Ponceau's solution, i am getting distinct bands at higher molecular weight region but not getting anything below 35 kD region. i'm approximately loading 25 micro gram of protein. i have confirmed ...

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... and Epicenter. I am looking forward to try those. I noticed that on the gel I ran for my genomic extract that there was much more intensity of bands that were very small (~50-400)bp compared to larger in tact fragments present. I think that this might have something to do with whats going on. ...

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