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Q bands

Q bands

(Science: cell biology) The alternating bright and dull fluorescent bands seen on chromosomes under ultraviolet light after the chromosomes are stained with quinacrine. The Q stands for quinacrine, an agent used as an antimalarial agent and, in the laboratory, as a fluorescent dye.


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plasmid extraction on 1.5% agarose gel

So I have two bands, the smaller that is around 8-9kb based on the marker ( my plasmids should be 8-9 kb), what is the upper band? Also I have three plasmids and I did this in a set of four replicates

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by cah2010
Wed Dec 18, 2013 2:23 am
 
Forum: Molecular Biology
Topic: plasmid extraction on 1.5% agarose gel
Replies: 5
Views: 1569

electrophoretic mobility shift assay (EMSA)

... EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, there was a bromophenol ...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1151

Gel Electrophoresis Help!

... or not. what are the restriction enzymes you are using? did you check if your vector contains multiple sites of the enzymes you are using? The bands are way up, higher than 10k bp..

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by bravebeaker
Thu Aug 29, 2013 7:14 am
 
Forum: Molecular Biology
Topic: Gel Electrophoresis Help!
Replies: 3
Views: 1299

Gel Electrophoresis Help!

So I did a double digest for subcloning and I didn't get two distinct bands when I ran the gel. Can someone interpret the gel pattern for me?

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by cks123
Tue Aug 27, 2013 4:25 pm
 
Forum: Molecular Biology
Topic: Gel Electrophoresis Help!
Replies: 3
Views: 1299

Re: Finding the net charge of amino acid

I have difficulty in evaluating the bands sspecially lower than 500 bp.

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by Richard02
Mon Jul 08, 2013 6:58 am
 
Forum: Molecular Biology
Topic: Finding the net charge of amino acid
Replies: 19
Views: 51239
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