Dictionary » P » Pyrophosphate



(Science: chemistry) Two phosphate groups linked by esterification. Released in many of the synthetic steps involving nucleotide triphosphates (e.g. Protein and nucleic acid elongation). Rapid cleavage by enzymes that have high substrate affinity ensures that these reactions are essentially irreversible.

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principle of error-prone PCR???

... to my knowledge they always provide magnesium, but there are proteins which work better with one or another (e.g. pyrophosphatase is specific for pyrophosphate with one metal while with the other it can cleave also other substrates as ATP etc.), the reason probably being the size of the metal ...

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by JackBean
Tue Sep 30, 2014 8:19 am
Forum: Molecular Biology
Topic: principle of error-prone PCR???
Replies: 5
Views: 2475

BCYE agar plates and legionella culture

... and modify surface tension. ACES Buffer is added to maintain the proper pH for optimal growth. -Ketoglutarate stimulates organism growth. Ferric Pyrophosphate supplies iron. Agar is the solidifying agent. BCYE Agar is supplemented with L-Cysteine, an essential amino acid incorporated to satisfy ...

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by LedBulb900Lumen
Thu Jan 05, 2012 2:37 pm
Forum: Microbiology
Topic: BCYE agar plates and legionella culture
Replies: 3
Views: 4058

ApoE Lipidation in Alzheimer's Disease

... that is attached to proteins. in the case of attached fatty acids i am pretty sure the bond is an ester bond. in the case of farnesyl pyrophosphate i am guessing the resulting bond is also an ester bond, and that the pyrosphate is hydrolyzed in the process (although i haven't actually ...

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by MrMistery
Sat Sep 19, 2009 3:14 pm
Forum: Molecular Biology
Topic: ApoE Lipidation in Alzheimer's Disease
Replies: 2
Views: 2576

The Fiber Disease

... and Sephadex G-100 columns, were comparatively studied with both enzymatic preparations. The effect of sodium diethyldithiocarbamate, sodium pyrophosphate, dithiothreitol, temperature, pH and O2, as well as the chromatographic behavior, would suggest that structural differences in the PCL ...

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by London
Thu Dec 28, 2006 6:33 am
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6244975

biochem exercise

... --> e) Glycogen(n) + PO4 --> f) Glycogen(n) + UDP-Glucose --> g) Malonyl CoA + ACP --> h) Palmytoil ACP + H2O --> i) Dimethylalyl pyrophosphate + isopentenyl pyrophosphate --> j) ADP + PO4 -->

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by kabuto
Wed Oct 25, 2006 3:41 am
Forum: General Discussion
Topic: biochem exercise
Replies: 2
Views: 2612
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