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Puromycin

puromycin

(Science: drug) An antibiotic that acts as an aminoacyl tRNA analogue. Binds to the A site on the ribosome, forms a peptide linkage with the growing chain and then causes premature termination.


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293 vs 293TN cells (for controls) in WB Analysis

... may code for Neomycin resistance in the cDNA, so we will use 293 since 293TN are Neomycin resistant and we can not select. Other collections have puromycin resistance, so we will use 293TN cells since they have higher transfection efficiencies and only Neomycin resistant. We would like to always ...

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by scsurfer
Tue Jun 29, 2010 11:31 pm
 
Forum: Cell Biology
Topic: 293 vs 293TN cells (for controls) in WB Analysis
Replies: 0
Views: 2029

question about shRNA knocking down

... by RNA PoII III) has been hypermethylated in LNCaP cells but i doubt that since i used very early passage cells after drug selection (2.0 ug/ml puromycin) for the western blot analysis, so from the point of kinetics that does not make sense. They also mentioned that maybe due to the processing ...

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by zjhzlk
Fri Nov 28, 2008 5:49 pm
 
Forum: Molecular Biology
Topic: question about shRNA knocking down
Replies: 1
Views: 2190

problem with retroviral infection of MEFs

... -80C until usage. Day 4. Morning: change medium for infected MEFs. Day 5. Split infected MEFs at 1:2 or 1:3 for either NO drug selection or 2ug/ml puromycin selection On Day 3 afternoon, during the 2nd infection, I could see MEFs change their morphology(become more flat) and some MEFs look like ...

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by wantz722
Tue Jul 08, 2008 4:36 am
 
Forum: Cell Biology
Topic: problem with retroviral infection of MEFs
Replies: 1
Views: 3759

How to select stably transfected phoenix cells

... the stably transfected Phoenix cells in order to produce hight titer supernatants, but the problem is that my retroviral construct do not carry puromycin resistance, only carries GFP so, how can I select for the stably transfected Phoenix cells? Is the retroviral vector integrated into the phoenix ...

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by sotadecopas
Tue Jul 01, 2008 12:12 pm
 
Forum: Molecular Biology
Topic: How to select stably transfected phoenix cells
Replies: 0
Views: 1468


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