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Dictionary » P » Purify

Purify

purify

1. To make pure or clear from material defilement, admixture, or imperfection; to free from extraneous or noxious matter; as, to purify liquors or metals; to purify the blood; to purify the air.

2. Hence, in figurative uses: To free from guilt or moral defilement; as, to purify the heart. And fit them so Purified to receive him pure. (milton)

To free from ceremonial or legal defilement. And Moses took the blood, and put it upon the horns of the altar, . . . And purified the altar. (Lev. Viii. 15) Purify both yourselves and your captives. (Num. Xxxi. 19)

To free from improprieties or barbarisms; as, to purify a language.

Origin: F.purifier, L. Purificare; purus pure _ -ficare (in comp) to make. See Pure, and -fy.


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qPCR

... extracted RNA --> DNase treatment --> RT to synthesize cDNAs 2. use same primers to amplify your target by PCR 3. extract bands from gel and purify target DNA 4. use the purified DNA for making a standard curve. 5. runa a qPCR

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by bravebeaker
Wed Dec 26, 2012 4:47 am
 
Forum: Molecular Biology
Topic: qPCR
Replies: 2
Views: 529

BAP, brassinolide, triacontanol, Amino, kelp foliar question

... do not know what is isoprenoid cytokinins. Are they some types of aromatic substances? Ester of some sort. It must be an almost impossible job to purify and identify those several proteins involved in their metabolism without advanced equipments. Do you use a PCR machine? I have not the slightest ...

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by Maton
Mon Dec 24, 2012 11:15 am
 
Forum: Botany Discussion
Topic: BAP, brassinolide, triacontanol, Amino, kelp foliar question
Replies: 15
Views: 11720

BAP, brassinolide, triacontanol, Amino, kelp foliar question

I'm no expert, but are you sure you're talking about 30% purify? What are the 70%? Or is it 30% solution?

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by JackBean
Sat Dec 22, 2012 1:06 pm
 
Forum: Botany Discussion
Topic: BAP, brassinolide, triacontanol, Amino, kelp foliar question
Replies: 15
Views: 11720

DNA extraction from stool

Hello, I need to purify DNA from animals and use my own protocol instead of buying kits all the time. Can anybody suggest a good protocol to purify clean DNA which can be used in PCRs? Thanks.

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by sevdakara
Thu Dec 20, 2012 6:53 pm
 
Forum: Molecular Biology
Topic: DNA extraction from stool
Replies: 0
Views: 274

Re:

... initially had 4 sets of primers. I was going to amplify 4 overlapping regions of the gene, run them on a gel, cut out the bands of expected size, purify them, and eventually sequence and ligate them together to generate the full length gene which I would then clone and amplify. However, there ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 882
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