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Purify

purify

1. To make pure or clear from material defilement, admixture, or imperfection; to free from extraneous or noxious matter; as, to purify liquors or metals; to purify the blood; to purify the air.

2. Hence, in figurative uses: To free from guilt or moral defilement; as, to purify the heart. And fit them so Purified to receive him pure. (milton)

To free from ceremonial or legal defilement. And Moses took the blood, and put it upon the horns of the altar, . . . And purified the altar. (Lev. Viii. 15) Purify both yourselves and your captives. (Num. Xxxi. 19)

To free from improprieties or barbarisms; as, to purify a language.

Origin: F.purifier, L. Purificare; purus pure _ -ficare (in comp) to make. See Pure, and -fy.


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Mitochondrial Research

I have not done any mitochondrial studies 1. purify the mitochondria from normal cells check if ETC is going on well by adding succinate 2. divide in to three groups 3. group 1 normal ETC, 2 standard modifier 3 your compound 4. compare the results

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by balamurugan3
Fri Aug 16, 2013 9:52 pm
 
Forum: General Discussion
Topic: Mitochondrial Research
Replies: 1
Views: 753

protein fractionation

There are several ways how to purify your protein (bulk fractionation, liquid chromatography, electrophoresis). Best is if you have previously published protocol, at least of similar protein. If you have any, you just have to try different ...

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by JackBean
Thu May 09, 2013 2:03 pm
 
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 2064

protein fractionation

... of the transcriptional regulator, it results in secretion of a protein factor in the media, which has interesting biological roles. My aim is to purify this unknown factor present in the growth media and to identify it by Mass Spec. But the problem is I am not familiar with the fractionation ...

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by salmonela
Thu May 09, 2013 11:17 am
 
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 2064

qPCR

... extracted RNA --> DNase treatment --> RT to synthesize cDNAs 2. use same primers to amplify your target by PCR 3. extract bands from gel and purify target DNA 4. use the purified DNA for making a standard curve. 5. runa a qPCR

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by bravebeaker
Wed Dec 26, 2012 4:47 am
 
Forum: Molecular Biology
Topic: qPCR
Replies: 2
Views: 1793

BAP, brassinolide, triacontanol, Amino, kelp foliar question

... do not know what is isoprenoid cytokinins. Are they some types of aromatic substances? Ester of some sort. It must be an almost impossible job to purify and identify those several proteins involved in their metabolism without advanced equipments. Do you use a PCR machine? I have not the slightest ...

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by Maton
Mon Dec 24, 2012 11:15 am
 
Forum: Botany Discussion
Topic: BAP, brassinolide, triacontanol, Amino, kelp foliar question
Replies: 15
Views: 19773
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