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Dictionary » P » Purify

Purify

purify

1. To make pure or clear from material defilement, admixture, or imperfection; to free from extraneous or noxious matter; as, to purify liquors or metals; to purify the blood; to purify the air.

2. Hence, in figurative uses: To free from guilt or moral defilement; as, to purify the heart. And fit them so Purified to receive him pure. (milton)

To free from ceremonial or legal defilement. And Moses took the blood, and put it upon the horns of the altar, . . . And purified the altar. (Lev. Viii. 15) Purify both yourselves and your captives. (Num. Xxxi. 19)

To free from improprieties or barbarisms; as, to purify a language.

Origin: F.purifier, L. Purificare; purus pure _ -ficare (in comp) to make. See Pure, and -fy.


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Purified Enzyme

... nowhere. There is usually a cell (or more)around with plenty of other enzymes and molecules in it. So if you want to study One enzyme, you need to purify it, that is to selectively remove it from the rest of the cell to get a solution of only one molecule, the enzyme.

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by canalon
Thu Sep 03, 2009 2:53 pm
 
Forum: Molecular Biology
Topic: Purified Enzyme
Replies: 1
Views: 31

I get short smears trying to amplify tomato DNA, pleaseHelp

... on the yield, than the creation of some kind of fuzzy band... Although I am more a bacteria person I know that plant DNA is notoriously hard to purify correctly as the large number of polysaccharides and multiples organic acids can be co-purified with the DNA. I am currently working with algae ...

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by canalon
Sat Jul 25, 2009 1:25 am
 
Forum: Molecular Biology
Topic: I get short smears trying to amplify tomato DNA, pleaseHelp
Replies: 5
Views: 170

Re: Diversifying selection

... and resources but conversely it is caused by them, and it's direction can be changed by them. NS only acts on what is already there--it can "purify" a trait in a population, causing it to be predominant, or delete traits (not always permanently). A gene must be there first before it ...

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by AFJ
Sat Jul 11, 2009 5:43 am
 
Forum: Evolution
Topic: Diversifying selection
Replies: 15
Views: 451

Some problems of DNA recovery from native PAGE gel

My protocol of the PAGE gel running and DNA recovery is as follows, 1. 20% native PAGE gel (The DNA i want to purify is around 20bp dsDNA + 10 bp ssDNA ) 2. running at 250 v for 30mins, 3. stain and cut the desired band; 4. cursh the gel by yellow tips into small gains; 5. add ...

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by wusilinqi
Thu May 14, 2009 5:20 am
 
Forum: Genetics
Topic: Some problems of DNA recovery from native PAGE gel
Replies: 1
Views: 550

constuction of a full length gene

... restriction enzymes, so that the site on the end of one partial copy matches the site at the beginning of the next one. Then cut them, column-purify, and ligate.

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by MrMistery
Thu Apr 30, 2009 12:04 am
 
Forum: Molecular Biology
Topic: constuction of a full length gene
Replies: 1
Views: 237
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