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Purification

purification

(Science: chemistry) A central part of downstream processing. Large-scale purification methods are used to take a crude fermentation supernatant or cell homogenate and isolate the product from it in a fairly pure form.


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Re: Why dialyse DMEM before protein purification (Ni-Affinity)?

I attempted to purge my protein from the unit society supernatant. I transfected my units with my develop to prepare my protein in the supernatant. I gathered the supernatant around the range of 1 litre and after dialysis I subjected my supernatant to cleaning yet I don't get my proteins at the clos...

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by sullivan
Mon Jul 22, 2013 12:28 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2592

protein fractionation

... any, you just have to try different approaches and detect both your protein (enzymatic activity?, blotting...) and total protein and determine purification fold. Great for you would be the first chapters from methods in Enzymology 463 http://www.sciencedirect.com/science/bookseries/00766879/463

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by JackBean
Thu May 09, 2013 2:03 pm
 
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 2666

Campylobacter RFLP troubleshooting

... cut it with RE for what exactly? Just at the ends to make sticky ends for consequent cloning? And I guess you use your PCR reaction without any purification?

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by JackBean
Sun May 05, 2013 3:54 pm
 
Forum: Molecular Biology
Topic: Campylobacter RFLP troubleshooting
Replies: 4
Views: 1887

Nuclear protein purification

Hi all, During nuclear protein purification I came across the step where I need to add 5M NaCl slowly with shaking until I adjust the molarity to 2M NaCl, how many mls do I need to add? Honestly I tried to tackle the problem in different ...

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by bravebeaker
Mon Mar 18, 2013 7:49 am
 
Forum: Molecular Biology
Topic: Nuclear protein purification
Replies: 4
Views: 1634

UWM is seeking testers for a new protein expression kit

... Owen, H.A.; and M.L.P. Collins. 2010. A new system for heterologous expression of membrane proteins: Rhodospirillum rubrum. Protein Expression and Purification, 70: 88-94. http://www4.uwm.edu/letsci/biologicalsciences/facultystaff/collins/upload/Butzin_et_al_2010.pdf

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by ejhuwmfdn
Thu Feb 14, 2013 3:05 pm
 
Forum: Molecular Biology
Topic: UWM is seeking testers for a new protein expression kit
Replies: 0
Views: 2035
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