Purification

Hello, I am doing PPARs (a nuclear receptor, a nuclear protein) purification by using PPARs transformed E.coli. But by now I have not got any PPARs. I do not know why. If there is anyone who knows about this or has done some experiments about this, please ...

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... http://jvdi.org/cgi/reprint/13/2/162.pdf and I am pretty sure that this method can be adapted for RNA (use the lysis conditions then do a trizol purification instead of the phenol choloroform): http://jvdi.org/cgi/reprint/13/2/162.pdf

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... number of polysaccharides and multiples organic acids can be co-purified with the DNA. I am currently working with algae samples, and finding a purification method that would get rid of the inhibitors took me a while. There are some kits that are special for plants or have modification for plants ...

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... min; 7. take out the supernatant, and the DNA should be in it. The problem is that when I use nanodrop to measure the concentraion and check the purification, i found that only 10% DNA is recovered, and the product is not pure. Please help me to find the mistakes. Thanks.

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... should be reconsidered: - Many cheese are acidic, and DNA can be damaged even by weak acid. - Cheese is fat, and that is not ideal for DNA purification - What size of sample are you using, maybe it is too small ? If you can have access to scientific journal I suggest you start by reviewing ...

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