Dictionary » P » Pulse chase

Pulse chase

pulse chase

(Science: biochemistry) An experimental protocol used to determine cellular pathways, such as precursor product relationships.

A sample organism, cell or cellular organelle), is exposed for a relatively brief time to a radioactively labelled molecule, the pulse. It is then replaced with an excess of the unlabelled molecule, the chase (cold chase). The sample is then examined at various later times to determine the fate of radioactivity incorporated during the pulse.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Enzymes

It has been done for many specific proteins. One good method is pulse-chase analysis. http://en.wikipedia.org/wiki/Pulse-chase_analysis

See entire post
by jonmoulton
Wed Aug 15, 2012 4:50 pm
 
Forum: Molecular Biology
Topic: Enzymes
Replies: 23
Views: 16996

Cell cycle

This is a classic pulse chase experiment. At t=0 the marker is inserted and then you follow its fate over time. In this case you need ...

See entire post
by canalon
Wed Mar 09, 2011 8:46 pm
 
Forum: Cell Biology
Topic: Cell cycle
Replies: 2
Views: 2640

I need answers to the following questions...

... a. the cells might not have been labeled at all. b. the time from pulse to first cells entering mitosis would probably have been longer. c. ... above would likely have occurred. 5. In George Palade's classic pulse-chase experiments, the importance of performing the chase as opposed to continuous ...

See entire post
by WiseIdiot
Sat Oct 18, 2008 5:55 pm
 
Forum: Cell Biology
Topic: I need answers to the following questions...
Replies: 3
Views: 13072

Pulse chase plot --cant understand how to decipher these???

The idea of the pulse-chase experiment is to allow you to follow incorporated label over time to see where it goes. Most often they ...

See entire post
by blcr11
Fri Nov 16, 2007 12:39 pm
 
Forum: Molecular Biology
Topic: Pulse chase plot --cant understand how to decipher these???
Replies: 2
Views: 1956

Pulse chase plot --cant understand how to decipher these???

Can sum1 pls help with these graphs. I understand these plots when its just 2 compounds, but what is happening with 4, and what steps do you take when looking at the graph to decipher what is happening at the mplecular level? thanks for any help! example.. //courses.washington.edu/bioc440/lectures/2...

See entire post
by ahjse19
Fri Nov 16, 2007 2:50 am
 
Forum: Molecular Biology
Topic: Pulse chase plot --cant understand how to decipher these???
Replies: 2
Views: 1956
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 8,700 times. 
What links here | Related changes | Permanent link