Pulse chase

pulse chase

(Science: biochemistry) An experimental protocol used to determine cellular pathways, such as precursor product relationships.

A sample organism, cell or cellular organelle), is exposed for a relatively brief time to a radioactively labelled molecule, the pulse. It is then replaced with an excess of the unlabelled molecule, the chase (cold chase). The sample is then examined at various later times to determine the fate of radioactivity incorporated during the pulse.


Please contribute to this project, if you have more information about this term feel free to edit this page



This page was last modified on 3 October 2005, at 21:16. This page has been accessed 9,717 times. 
What links here | Related changes | Permanent link