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Protoplast

Definition

noun, plural: protoplasts


Plant, bacterial or fungal cell with the cell wall removed using either mechanical or enzymatic means.

The first from which all subsequent forms are derived; prototype; archetype


Supplement

Word origin: French protoplaste, from Old French, the first man, from Late Latin prōtoplastus, from Greek prōtoplastos : prōto-, proto- + plastos, formed, molded.

Related forms: protoplastic (adjective).

Compare: spheroplast.
See also: cytoplasm, cell wall.


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TCA preipitation

... 5000rpm for 10 mins Remove Supernatant and resuspend pellet in PBS Spin at 5000rpm for ten mins Discard Supernatant and resuspend pellet in 1 mL protoplast buffer. Incubate for 3hours at 37C spin at 13K for 15mins store supernatant at -20C So the supernatant will contain cell wall and the insoluble ...

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by biology_06er
Wed Feb 27, 2013 3:25 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 2494

The same old silly question about molarities

... I have a question and I would like you to help me figure it out. Sometimes we read about solution contents for example the enzyme solution used in protoplast isolation, well in the paper its written like this 20 mM MES 0.4 M mannitol 20 mM KCl 10 mM CaCl2 0.1% BSA 1.5% Cellulase and so on... I ...

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by bravebeaker
Wed Dec 26, 2012 3:35 am
 
Forum: Molecular Biology
Topic: The same old silly question about molarities
Replies: 3
Views: 1650

dot blot--protoplasts

A question regarding protoplasts in a dot blot..my protein of interest is in the protoplast which I has isolated and I have been told to do a titration/dilution of it...so I was told do 4x 10 fold diltuin...does this mean if I started ...

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by biology_06er
Wed Jan 11, 2012 1:35 am
 
Forum: Molecular Biology
Topic: dot blot--protoplasts
Replies: 1
Views: 669

Protoplast Isolation - first step - enzyme digestion

Hello all, I have been reading lots of papers about protoplast isolation. I found that most of the papers but not all say that enzyme digestion is in the dark. Why should it be in the dark? i have been trying to get an explanation but cant find any. ...

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by bravebeaker
Fri Dec 16, 2011 9:02 am
 
Forum: Molecular Biology
Topic: Protoplast Isolation - first step - enzyme digestion
Replies: 0
Views: 960

Protoplast Isolation

Hello there forum. I am new here and studying under the HL IB programme. I have chosen to do an extended essay in biology, obviously, and I am requesting some assistance on the topic. My experiment involves the isolation of protoplasts from lettuce leaf. The second part of the experiment is the fusi...

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by ev350
Thu Sep 08, 2011 8:23 pm
 
Forum: Cell Biology
Topic: Protoplast Isolation
Replies: 2
Views: 6224
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