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Protocols

protocol

A formula, a treatment recipe.


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Re: When is it okay to call a scientist a whore?

... many more posts about this. I don't feel the entire site should suffer for one independent editor's (egregious) error. While I'm not sure of your protocols, you might consider taking this up your supervisory chain to get someone to post an official response that an inquiry is pending. That won't ...

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by HanziBeldam
Sun Oct 13, 2013 8:03 am
 
Forum: General Discussion
Topic: When is it okay to call a scientist a whore?
Replies: 79
Views: 74811

Overexpression

... of protocol are designed for cell line macrophage but not for primary cells as BMDM. Is everyone know if there are some differences between the protocols ? Thank you so much for your help Aurélie

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by aureliegineste
Wed Sep 04, 2013 7:48 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1470

calcium measurement- help!!

Hello everyone, i'm looking for ideas and protocols how to measure Ca+2 conc. by using the fluorofore Flue-3 (single exitation wavelenght). I understand the equation with Kd, Fmin, Fmax etc. but don't know exactly how to do it: what conc. should ...

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by reulita10
Tue Jul 16, 2013 12:35 pm
 
Forum: Molecular Biology
Topic: calcium measurement- help!!
Replies: 0
Views: 453

calcium measurement- help!!

Hello everyone, i'm looking for ideas and protocols how to measure Ca+2 conc. by using the fluorofore Flue-3 (single exitation wavelenght). I understand the equation with Kd, Fmin, Fmax etc. but don't know exactly how to do it: what conc. should ...

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by reulita10
Tue Jul 16, 2013 12:34 pm
 
Forum: General Discussion
Topic: calcium measurement- help!!
Replies: 0
Views: 456

protein fractionation

... by Mass Spec. But the problem is I am not familiar with the fractionation methods. Looking through the literature, I could see that many different protocols are being employed out of which I don’t know which the best is. I like your suggestion with regard to a good standard protocol for identifying ...

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by salmonela
Thu May 09, 2013 11:17 am
 
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 2680
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