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Protocol

protocol

A formula, a treatment recipe.


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Primer problem

Hi I designed a specific primers and blast it to amplify my gene. this primer amplifies a gDNA of the gene. I used dreamtaq kit and its protocol but unfortunately the expected product (2054 bp) was not there and what I got is several bands below and above my expected product size. Then I have ...

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by roniadam
Sat Sep 05, 2009 3:10 am
 
Forum: Molecular Biology
Topic: Primer problem
Replies: 1
Views: 15

cell cloning by limiting dilution

hi every body does any body did limiting dilution for creating single cell clones? is it a straightforward way or not? any help or protocol will be appreciated best

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by lalehr
Fri Sep 04, 2009 10:22 am
 
Forum: Cell Biology
Topic: cell cloning by limiting dilution
Replies: 1
Views: 27

Isolating algae pigments - Paper chromatography?

... use pretty much any organic solvent with good results. Here is a link http://www.monsanto.com/pdf/responsibility/chlorophyll_extraction.pdf to a protocol describing chlorophyll extraction using isopropanol. Cheers, Andrei

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by MrMistery
Tue Sep 01, 2009 1:40 pm
 
Forum: Botany Discussion
Topic: Isolating algae pigments - Paper chromatography?
Replies: 1
Views: 37

Injection of pellets

I have to inject a treatment pellets to test animals in the neck, as described here: http://www.protocol-online.org/forums/index.php?showtopic=9090 Does someone have a tested method on how to do it ?

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by itamo
Mon Aug 24, 2009 4:20 am
 
Forum: General Discussion
Topic: Injection of pellets
Replies: 0
Views: 51

[molecular bio]calculation

1. A reaction has been design to be carried out in 4ml total volume. It requires a final phenylalanine concentration of 5mM. If the protocol allows 50 ul for the phenylalanine addition, what concentration would you make up the stock solution? 2.A particular cell line will grow well in a ...

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by scholesmu
Sat Aug 15, 2009 9:48 am
 
Forum: Molecular Biology
Topic: [molecular bio]calculation
Replies: 3
Views: 122
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