Protocol

Hi I designed a specific primers and blast it to amplify my gene. this primer amplifies a gDNA of the gene. I used dreamtaq kit and its protocol but unfortunately the expected product (2054 bp) was not there and what I got is several bands below and above my expected product size. Then I have ...

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hi every body does any body did limiting dilution for creating single cell clones? is it a straightforward way or not? any help or protocol will be appreciated best

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... use pretty much any organic solvent with good results. Here is a link http://www.monsanto.com/pdf/responsibility/chlorophyll_extraction.pdf to a protocol describing chlorophyll extraction using isopropanol. Cheers, Andrei

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I have to inject a treatment pellets to test animals in the neck, as described here: http://www.protocol-online.org/forums/index.php?showtopic=9090 Does someone have a tested method on how to do it ?

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1. A reaction has been design to be carried out in 4ml total volume. It requires a final phenylalanine concentration of 5mM. If the protocol allows 50 ul for the phenylalanine addition, what concentration would you make up the stock solution? 2.A particular cell line will grow well in a ...

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