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Protocol

protocol

A formula, a treatment recipe.


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Deproteinizing plasma?

Hello! Here is a protocol currently used by a core facility for the deproteinization of small volumes of mouse plasma for measurement of plasma radiolabeled glucose: 1) Add 10 uL of plasma into 20 uL of dd water. 2) Add 25 uL of BaOH ...

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by ChristopherC4rm34n
Tue Sep 09, 2014 6:15 pm
 
Forum: Physiology
Topic: Deproteinizing plasma?
Replies: 10
Views: 9461

mycoplasma testing

... as a second method. not sure how often and what lines I need to test (we have fibroblasts and neuronal cultures). does anyone have a example of a protocol that is used in his lab of what specimens to test and at what point of the culture? If so, that would be realy helpful. THANK YOU IN ADVANCE, ...

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by revi
Tue Feb 25, 2014 11:03 am
 
Forum: Molecular Biology
Topic: mycoplasma testing
Replies: 0
Views: 726

Re: centrifugation of hemocytes

Before you wash the cells, can you see any pellet? Wash gently, add your final PBS and pipet to resuspend. Your protocol sounds good to me.

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by jonmoulton
Tue Jan 28, 2014 3:53 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2918

centrifugation of hemocytes

... flat-bottom and round bottom). I took the supernatant off and tried to resuspend the cells with PBS and then performed my classical viability protocol… But the cells have gone… These velocities are the one used in articles… and i can't increase the velocity of centrifugation because i want ...

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by Thom
Mon Jan 27, 2014 6:41 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2918

Somatic Fusion and Reproduction

... tropical genetics? And would this hybrid be able to sexually reproduce with clones of itself? Supposing that this does work, what is the naming protocol for somatic hybrids? Could I choose something, which would relate to the Latin name of the parent plants (apple x potato malus tater ), or ...

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by Thomas9666
Thu Jan 09, 2014 5:15 pm
 
Forum: Cell Biology
Topic: Somatic Fusion and Reproduction
Replies: 0
Views: 1952
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