Protocol

... me I would have three components by the end of it, the soluble, insoluble and something else!!! (the isolated proteins??) arrg I have looked at a protocol for a cell wall extraction. Here is a simplified version below Wait till OD 0.6 Spin at 5000rpm for 10 mins Remove Supernatant and resuspend ...

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Does anyone have a protocol for ELISA? Thanks

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... Our original plan is to isolate the protein using trizol extraction reagent, then run an ELISA on HIF 1 alpha. However, I am worried that the protocol may not work. I haven't heard of trizol being used for a specific protein extraction, and HIF 1 alpha seems to be favorably isolated with western ...

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Does anyone have areliable protocol for thawing cells from frozen stock? thanks

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Hello, I need to purify DNA from animals and use my own protocol instead of buying kits all the time. Can anybody suggest a good protocol to purify clean DNA which can be used in PCRs? Thanks.

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