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Protocol

protocol

A formula, a treatment recipe.


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RNA isolation from brain sections

... microtome (20 um to 200 um) and cut out particular brain regions to extract RNA. So, here are some questions: 1) any suggested RNA isolation kits/protocols for brain 2) suggestions for such small volumes of RNA 3) viability of RNA sectioned in cold buffer on a vibratome Overall I'd love to get ...

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by protoemics
Fri Mar 20, 2015 2:19 am
 
Forum: General Discussion
Topic: RNA isolation from brain sections
Replies: 0
Views: 37

Ligase reaction problem

... I uploded an image you can see below. Here, I want to ask whether it is enough base lenght for ligase reaction via thermostable ligase in 63 C (Protocol: 5 cycle at 95 C-30 s and 63 C-4 min) I think I take cross reaction because of this dimer but I am not sure. Is it possible to discard this ...

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by Molbio34
Thu Feb 26, 2015 8:27 am
 
Forum: Molecular Biology
Topic: Ligase reaction problem
Replies: 0
Views: 323

separation problem with GelRed

... a lower percentage gel. 4. Change the running buffer. TBE buffer has a higher buffering capacity than TAE buffer. 5. Try using the post-staining protocol if possible, especially if more than the recommended amount of DNA is required. For other questions regarding GelRed, check out Biotium’s FAQ ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19576

Deproteinizing plasma?

Hello! Here is a protocol currently used by a core facility for the deproteinization of small volumes of mouse plasma for measurement of plasma radiolabeled glucose: 1) Add 10 uL of plasma into 20 uL of dd water. 2) Add 25 uL of BaOH ...

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by ChristopherC4rm34n
Tue Sep 09, 2014 6:15 pm
 
Forum: Physiology
Topic: Deproteinizing plasma?
Replies: 10
Views: 11931

mycoplasma testing

... as a second method. not sure how often and what lines I need to test (we have fibroblasts and neuronal cultures). does anyone have a example of a protocol that is used in his lab of what specimens to test and at what point of the culture? If so, that would be realy helpful. THANK YOU IN ADVANCE, ...

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by revi
Tue Feb 25, 2014 11:03 am
 
Forum: Molecular Biology
Topic: mycoplasma testing
Replies: 0
Views: 1096
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