Dictionary » P » Proteolysis

Proteolysis

proteolysis

(Science: cell biology) cleavage of proteins by proteases. Limited proteolysis occurs where proteins are functionally modified (activated in the case of zymogens) by highly specific proteases.


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use of a bacterial protease inhibitor cocktail with mammalia

... E-64, and pepstatin A (E.coli protease inhibitor cocktail) but it does not include the aprotinin or the leupeptin inhibitors. Is there a risk of proteolysis in a mammalian cell lysate without these latter 2 inhibitors? The specific cell types which we are using are: HUVEC (human umbilical vein ...

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by rpwdoc
Tue Aug 26, 2014 10:08 pm
 
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 0
Views: 98

URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

... Quite turbid, slightly cloudy, no skin NUTRIENT AGAR STREAK- Shape – Cocci, Yellow, 0.2-0.5m Glossy NUTRIENT AGAR STAB: Aerobic GELATIN STAB: No Proteolysis THIOGLYCOLLATE MEDIUM- Anaerobic SEMI SOLID (Sloppy) Agar – Anaerobic BLOOD AGAR PLATE: B-Haemolysis HUGH AND LEIFSON’S O/F TEST: Negative ...

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by microbiologyhelp
Wed Nov 20, 2013 6:09 pm
 
Forum: Microbiology
Topic: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!
Replies: 6
Views: 5655

Re:

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS? sorry, but I do not know what ...

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by Bio2013
Sat Feb 16, 2013 8:48 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3274

Western Blot

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?

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by JackBean
Mon Feb 11, 2013 3:31 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3274

Re: Enzymes

... but damage accumulates and they are eventually broken down. This page might help the system make more sense. http://en.wikipedia.org/wiki/Proteolysis

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by jonmoulton
Tue Aug 07, 2012 3:32 pm
 
Forum: Molecular Biology
Topic: Enzymes
Replies: 23
Views: 16644
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