Dictionary » P » Proteolysis

Proteolysis

proteolysis

(Science: cell biology) cleavage of proteins by proteases. Limited proteolysis occurs where proteins are functionally modified (activated in the case of zymogens) by highly specific proteases.


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URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

... Quite turbid, slightly cloudy, no skin NUTRIENT AGAR STREAK- Shape – Cocci, Yellow, 0.2-0.5m Glossy NUTRIENT AGAR STAB: Aerobic GELATIN STAB: No Proteolysis THIOGLYCOLLATE MEDIUM- Anaerobic SEMI SOLID (Sloppy) Agar – Anaerobic BLOOD AGAR PLATE: B-Haemolysis HUGH AND LEIFSON’S O/F TEST: Negative ...

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by microbiologyhelp
Wed Nov 20, 2013 6:09 pm
 
Forum: Microbiology
Topic: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!
Replies: 6
Views: 2604

Re:

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS? sorry, but I do not know what ...

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by Bio2013
Sat Feb 16, 2013 8:48 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 2586

Western Blot

Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?

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by JackBean
Mon Feb 11, 2013 3:31 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 2586

Re: Enzymes

... but damage accumulates and they are eventually broken down. This page might help the system make more sense. http://en.wikipedia.org/wiki/Proteolysis

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by jonmoulton
Tue Aug 07, 2012 3:32 pm
 
Forum: Molecular Biology
Topic: Enzymes
Replies: 23
Views: 14751

Removing MBP tag

... too similar). Reaction was performed overnight at 4 °C Issues: 1) Only half of MBP-Fusionprotein is cut by PreScission. 2) Ignoring the incomplete proteolysis I performed the reaction onto the amylose resin column. In theory the cut protein has to be found in the flow-through whereas MBP and remaining ...

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by Papaver
Wed Aug 10, 2011 12:26 pm
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 7768
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