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Dictionary » P » Proteinase

Proteinase

proteinase --> endopeptidase

(Science: enzyme) An enzyme that cleaves protein at positions within the chain. Formally, the enzymes are peptidyl peptide hydrolases, more usually known as proteinases or proteolytic enzymes. Any enzyme that catalyzes the splitting of proteins into smaller peptide fractions and amino acids by a process known as proteolysis.


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how to increase DNA amount about 10 ug for digestion

Ok. Well I guess the proteinase K would not be very friendly to your RNAse either, would it? Besides, even of the RNAse is not degraded I wonder if SDS and other conditions are optimal for RNAse activity? As far as I can see, either ...

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by canalon
Mon Jun 30, 2008 3:39 pm
 
Forum: Molecular Biology
Topic: how to increase DNA amount about 10 ug for digestion
Replies: 12
Views: 1628

Re: Hair and DNA

... of the hair that contains actual living cells which, in turn, contain nDNA. There are many different extraction protocols to extract DNA, mainly proteinase K, NaOH, ethanol precipitaion, orgainc extraction, etc. Once the DNA is extracted, it is purified using a Microcon 100 concentrator or QIAGEN ...

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by AngieJ2118
Mon May 05, 2008 4:50 am
 
Forum: Physiology
Topic: Hair and DNA
Replies: 20
Views: 13752

pharmaceutical biotechnology

... antivenins, and venoms Blood, blood components, plasma derived products (for example, albumin, immunoglobulins, clotting factors, fibrin sealants, proteinase inhibitors), including recombinant and transgenic versions of plasma derivatives, (for example clotting factors), blood substitutes, plasma ...

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by kcnq1ot1
Tue Nov 27, 2007 2:47 pm
 
Forum: Molecular Biology
Topic: pharmaceutical biotechnology
Replies: 6
Views: 813

using cheek cell dna

... again. 4. remove 770 uL water. do not disturb the pellet. 5. add a little amount of chelex with a yellow tip. 6. vortex for 10 sec. 7. add 2 uL proteinase K and agitate. 8. incubate at 56 C for 5 minutes. 9. vortex for 10 seconds. 10. incubate in boiling water bath for 8 minutes. 11. vortex ...

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by sdekivit
Wed Aug 01, 2007 7:46 am
 
Forum: Molecular Biology
Topic: using cheek cell dna
Replies: 2
Views: 680

NEED IMMIDIATE HELP WITH RNA EXTRACTION!!

... RecoverAll Total Nucleic Acid Kit. According to the Ambion summary of their method, they are add something called “Isolation additive” after the proteinase K but before the ethanol precipitation step. They are a little vague as to what that “additive” is. The kit is supposedly optimized for recovering ...

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by blcr11
Thu Jul 19, 2007 8:13 pm
 
Forum: Molecular Biology
Topic: NEED IMMIDIATE HELP WITH RNA EXTRACTION!!
Replies: 2
Views: 682
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