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Proteinase

proteinase --> endopeptidase

(Science: enzyme) An enzyme that cleaves protein at positions within the chain. Formally, the enzymes are peptidyl peptide hydrolases, more usually known as proteinases or proteolytic enzymes. Any enzyme that catalyzes the splitting of proteins into smaller peptide fractions and amino acids by a process known as proteolysis.


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His-tagged proteins

Hi everybody, I have some doubts about the use of his-tagged HIV-1 proteinase for an inhibition assay. I have to test some potential inhibitors and I would like to perform a kinetic assay to obtain the percentage of inhbition and the IC50 values. Can the ...

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by claire84
Tue Jan 03, 2012 9:13 am
 
Forum: Cell Biology
Topic: His-tagged proteins
Replies: 2
Views: 1782

question about LCM

... And the thickness of the section? I use Arcturus PicoPure RNA Isolation Kit (#KIT0204) to isolate RNA from LCM cells, is it better to add proteinase K to the Extraction Buffer to help extraction since the proteinase K could help extract RNA by reversing the extensive crosslinking network ...

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by johnmay
Mon Oct 17, 2011 4:43 pm
 
Forum: Molecular Biology
Topic: question about LCM
Replies: 0
Views: 1149

DNA Extraction/Isolation

At the end of DNA extraction, heat at a temperature of 55 degrees Celsius is applied to Digestion buffer/Proteinase K solution. Then, at the end of DNA Isolation DNA/TE buffer solution is heated to 65 degrees Celsius. Why can't both reactions be heated at either 55 or 65 degrees ...

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by Melstmu2010
Thu Jun 30, 2011 8:31 pm
 
Forum: General Discussion
Topic: DNA Extraction/Isolation
Replies: 2
Views: 3226

proteinase inhibitor II

Hi, I'm doing a little research for a paper I'm writing on the proteinase inhibitor II (Pin2) gene found in Solanum lycopersicum (tomato plant). So far, I've uncovered various studies, most are much to involved for what I need. I’m looking for something ...

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by Thraxman
Sun Oct 24, 2010 12:28 am
 
Forum: Botany Discussion
Topic: proteinase inhibitor II
Replies: 0
Views: 1621

Vivantis Bacterial DNA extraction kit

... even environmental isolates). Usually because the cell wall is different is different and not properly degraded. In your kit they seem to rely on proteinase K, just like Qiagen, and in my experience it is far form good for even E. coli environmental isolates. There are alternative technologies ...

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by canalon
Fri May 28, 2010 1:14 pm
 
Forum: Molecular Biology
Topic: Vivantis Bacterial DNA extraction kit
Replies: 10
Views: 6210
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