Protein s

Hello, I'm trying to see the rate of degradation of a certain protein in 293 cells and i want to compare my results to known protein's half-life, for example actin or another abundant cellular protein. Does anyone have information regarding the half-lives ...

See entire post

... affinity chromatography , IMAC ). I wished to try it as one (or more) step(s) in my purification protocol and tried to study in the 2nd edition of Protein purification textbook, but it didn't work very well, or better said, it was kond of confusing: First of all, I found, that it might be usefull ...

See entire post

... mandatory research project this year. What I plan to do is attach a Fas (death receptor ligand) to a mesothelin antibody by binding the 2 with the protein crosslinker Glutaraldehyde. This ligand-antibody combination will then be administered to pancreatic cancer cells that overexpress mesothelin. ...

See entire post

when you guys keep on saying protein came first, do you mean peptide chains? Cause a fully functional protein has to fold with the tertiary structures. Only a few simple proteins will just form on their own. The complex enzymes usually ...

See entire post

... rat and mouse cDNAs in luciferase assay. The infected cells (in both rat and mouse cell lines) were selected on antibiotic. But when I look at the protein expression in the cells by Western blot, there seem to be no reduction. Of the sequences, at least 3 showed significant knockdown in Luciferase ...

See entire post