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Protein binding

protein binding

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.


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What makes dominant allele dominant?

... about expression. The difference can be anywhere from transcription to protein post-translational modifications. There can be mutation in the promotor changing significantly expression. If the binding of ribosome will be impaired, there will be much less of protein made. Single ...

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by JackBean
Thu Feb 27, 2014 5:20 pm
 
Forum: Genetics
Topic: What makes dominant allele dominant?
Replies: 7
Views: 3953

cAMP 2nd Messenger Pathway and Experiments

... 1: Cell membranes from cyc- cells were isolated and mixed with protein extracts from cells which exhibited adenylyl cyclase activity (cyc+ ... It interacts with GTP regulatory site to prohibit the GDP from binding. If ATP or GTP were eliminated from the reaction, it would not be ...

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by mkay28
Tue Sep 24, 2013 10:43 pm
 
Forum: Cell Biology
Topic: cAMP 2nd Messenger Pathway and Experiments
Replies: 2
Views: 2131

The question about TATA sequence flexibility

... I read this article about TATA-Binding Protein: Second, the protein uses specially-placed amino acids to interact with DNA bases. ...

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by Fantomas
Sat Aug 31, 2013 10:57 am
 
Forum: Molecular Biology
Topic: The question about TATA sequence flexibility
Replies: 1
Views: 1695

Re: nuclear DNA binding

... check against the chromosomal sequence. It's useful to know where the protein is interacting with the DNA. That doesn't eliminate the possibility that the protein also binds cytoplasmic DNA, but if there is nuclear DNA binding you can nail that down. You could also fluorescent-tag the protein ...

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by jonmoulton
Tue Jan 22, 2013 6:39 pm
 
Forum: Molecular Biology
Topic: nuclear DNA binding
Replies: 1
Views: 1873

nuclear DNA binding

Hi! I am wondering how I can demonstrate the association of a protein of interest with nuclear DNA, if nothing is known about the binding preferences at all. Is there a standard that I might not be aware of? I thought that ...

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by Balls
Tue Jan 22, 2013 9:03 am
 
Forum: Molecular Biology
Topic: nuclear DNA binding
Replies: 1
Views: 1873
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