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Protein binding

protein binding

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.


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western blotting of DNA-binding protein of mammalian cell

i am trying to immunoblot one of the DNA-binding protein.... first i have cultured the cells in 25 cm2 flask and collected the cell lysate using RIPA ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
 
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 431

Re: immunoglobulins

A protein of animal origin with known antibody activity. Immunoglobulins are major components ... figure.) Each class varies in its chemical structure and in its number of antigen-binding sites and adheres to and reacts only with the specific antigen for which it was produced.

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by RZachary1
Wed Jan 14, 2015 5:49 am
 
Forum: Cell Biology
Topic: immunoglobulins
Replies: 2
Views: 2333

I have got some BIG issues! forgive the long post

... at the ABO locus, catalyze the last step of the synthesis, while the protein coded by the O allele is non-functional; therefore, the acceptor ... 29000. They consits of two domains (1 and 1) forming the peptide- binding region (PBR) and two other immunoglobulin like domains (2 and 2) ...

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by saddy51
Sun Jan 04, 2015 10:39 am
 
Forum: Genetics
Topic: I have got some BIG issues! forgive the long post
Replies: 1
Views: 2066

Re: translation from DNA

That's a reasonable idea. I can imagine a ribosome directly binding to DNA to translate the DNA codons directly into protein. We can't really tell why that didn't happen, as much of the early development of life ...

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by jonmoulton
Mon Sep 29, 2014 3:03 pm
 
Forum: Molecular Biology
Topic: translation from DNA
Replies: 4
Views: 5450

Re: Any ideas for vaccine development or anti-viral drugs???

... DL, Nelle T, Iversen PL, Bavari S, Aman MJ. Involvement of Vacuolar Protein Sorting Pathway in Ebola Virus Release Independent of TSG101 Interaction. ... The discovery process involved identification of optimal transcript binding sites for PMO based RNA-therapeutics followed by screening for effective ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12827
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