Protein as

Your protein is crucial for myoblast survival?

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But the buffer in the new ladder should be the same as the others. The ladder is basically combining 2ul of each of the 9 dye-conjugated proteins (~18ul total). The dye-conjugated protein that I loaded separately and combined in the ladder are from the same solution. In addition, I loaded ...

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Hi there, I have to do a cell wall extraction on bacteria soon and my protein of interest is cell wall anchored. My supervisor told me to do TCA precipitation (haven't done before) but I assume it's too isolate the proteins within the cell wall?? He told me ...

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Hi everyone! I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% ...

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Typically you would use an antibody to bind to the protein of interest and then a secondary antibody carrying a fluorescent tag to detect the first antibody. The two-antibody approach is cheaper than making a custom-labelled fluorescent antibody for ...

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