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Dictionary » P » Protein ProteinDefinition noun, plural: proteins A molecule composed of polymers of amino acids joined together by peptide bonds. It can be distinguished from fats and carbohydrates by containing nitrogen. Other components include carbon, hydrogen, oxygen, sulphur, and sometimes phosphorus.
A protein is a linear polymer built from about 20 different amino acids. The type and the sequence of amino acids in a protein are specified by the DNA in the cell that produces them. This sequence of amino acids is essential since it determines the overall structure and function of a protein. A protein has several functions. It may serve as a structural material (e.g. keratin), as enzymes, as transporters (e.g. hemoglobin), as antibodies, or as regulators of gene expression. A protein may be classified based on its form and main functions: it can be a globular protein like most enzymes, fibrous protein which are for structural role; and membrane proteins that serve as receptors or channels for polar or charged molecule to pass through the cell membrane.
Related phrases: housekeeping protein, protein synthesis, carrier protein, membrane protein, plasma protein, integral membrane protein, coat protein, heat-shock protein, simple protein. See also: amino acid, enzyme. ![]()
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Protein ladder width increases down the gel?But the buffer in the new ladder should be the same as the others. The ladder is basically combining 2ul of each of the 9 dye-conjugated proteins (~18ul total). The dye-conjugated protein that I loaded separately and combined in the ladder are from the same solution. In addition, I loaded ...
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TCA preipitationHi there, I have to do a cell wall extraction on bacteria soon and my protein of interest is cell wall anchored. My supervisor told me to do TCA precipitation (haven't done before) but I assume it's too isolate the proteins within the cell wall?? He told me ...
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Protein ladder width increases down the gel?Hi everyone! I'm trying to construct a protein ladder, which are markers used to indicate the molecular weight of proteins when running a SDS-PAGE. I have conjugated several different molecular weight proteins with dyes and ran them on a 12% ...
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Re: Difference between ICC and IFTypically you would use an antibody to bind to the protein of interest and then a secondary antibody carrying a fluorescent tag to detect the first antibody. The two-antibody approach is cheaper than making a custom-labelled fluorescent antibody for ...
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