Proteases

... primed by random hexamers. Curiously, the same cDNA gives weird results when taken as template to amplify specific gene products: for some proteases, very bright bands. for some transcription factors highly expressed at the same time, no bands at all. for a housekeeping gene in the species ...

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... protease site between fusion protein and the peptide of interest (actually I have 2 constructs: with enterokinase and with factor Xa site). Both proteases seem to work, but both show some nonspecificity. I use tricine sds gel, that is for visualising small peptides, but I don’t get a band at ...

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... to answer it and list my questions): 15. You are determining the amino acid sequence of a protein isolated in the lab. You have used two different proteases to cleave the protein. These are the fragments after cleavage (all written with N terminus at the left and C terminus at the right) Protease ...

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... How does that make sense? I don't understand. i have never heard of proteoses in >10 years in the industry. Much more likely is you mean proteases. These are enzymes (usually proteins themselves) which snip or chew proteins. Enzymes in the stomach are good examples (e.g. trypsin, pepsin). ...

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... would this protein get into the urine'?? Ive been thinkin about this question..and i am thinkin that it could get excised by membrane-associated proteases releasing the soluble extracelular domain into the extracelular space of epithelial bladder tissue....but wondering ow it would find its way ...

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