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Proteases

proteases

enzymes that degrade protein molecules.


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PCR from cDNA question

... primed by random hexamers. Curiously, the same cDNA gives weird results when taken as template to amplify specific gene products: for some proteases, very bright bands. for some transcription factors highly expressed at the same time, no bands at all. for a housekeeping gene in the species ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
 
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 3081

MALDI; fusion protein cleaving from a peptide

... protease site between fusion protein and the peptide of interest (actually I have 2 constructs: with enterokinase and with factor Xa site). Both proteases seem to work, but both show some nonspecificity. I use tricine sds gel, that is for visualising small peptides, but I don’t get a band at ...

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by Valya
Wed Mar 28, 2012 8:42 pm
 
Forum: Molecular Biology
Topic: MALDI; fusion protein cleaving from a peptide
Replies: 6
Views: 2047

Edman Degradation Problem

... to answer it and list my questions): 15. You are determining the amino acid sequence of a protein isolated in the lab. You have used two different proteases to cleave the protein. These are the fragments after cleavage (all written with N terminus at the left and C terminus at the right) Protease ...

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by pinkerpikachu
Sun Oct 16, 2011 7:35 pm
 
Forum: Molecular Biology
Topic: Edman Degradation Problem
Replies: 1
Views: 3917

Re: Derived proteins

... How does that make sense? I don't understand. i have never heard of proteoses in >10 years in the industry. Much more likely is you mean proteases. These are enzymes (usually proteins themselves) which snip or chew proteins. Enzymes in the stomach are good examples (e.g. trypsin, pepsin). ...

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by merv
Sun Oct 02, 2011 4:29 pm
 
Forum: Molecular Biology
Topic: Derived proteins
Replies: 3
Views: 2900

How do receptor proteins get into urine??

... would this protein get into the urine'?? Ive been thinkin about this question..and i am thinkin that it could get excised by membrane-associated proteases releasing the soluble extracelular domain into the extracelular space of epithelial bladder tissue....but wondering ow it would find its way ...

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by bs07cp
Fri Apr 15, 2011 10:19 pm
 
Forum: Cell Biology
Topic: How do receptor proteins get into urine??
Replies: 1
Views: 1919
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