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Protease inhibitor

protease inhibitor

A drug that binds to and blocks hIV protease from working, thus preventing the production of new infectious viral articles.


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Amyloid beta (A4) precursor protein degradation products

... APP770. Compared with the other two isoforms, APP695 lacks the Kunitz protease inhibitor (KPI) region outside the cell. Neurons expressing APP695 subtypes in the cerebral ...

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by Anonymous
Tue Jun 12, 2012 3:11 am
 
Forum: General Discussion
Topic: Amyloid beta (A4) precursor protein degradation products
Replies: 1
Views: 6494

Re: Western Blot TroubleShooting

I harvest my samples using RIPA buffer + protease inhibitor on ice. I use a 6 well dish and I scrape the samples off on ice. I rotate the samples at 4C ...

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by mutant11
Mon Nov 21, 2011 3:11 am
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 3185

Western Blot TroubleShooting

... cell lines. Does anyone have any suggestions? -I use RIPA buffer with protease inhibitor to harvest the protein. I keep everything on ice. -I incubate in primary antibody ...

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by mutant11
Fri Nov 18, 2011 10:17 pm
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 3185

Removing MBP tag

... Here’re the facts: It’s a MBP-tag that should be removed. PreScission protease is used (I can’t use factor Xa because the size of Xa chains and ... well as on-column reaction. Further: reduced concentration of protease inhibitor, extended reaction time, added fresh protease after overnight incubation, ...

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by Papaver
Wed Aug 10, 2011 12:26 pm
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8561

MAOI supplier

... for MAOI? What is the suggested amount? Other than MAOI, I also add protease inhibitor cocktail. Is there anymore chemicals to be added to prevent degradation of serotonin? ...

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by trimethoprim
Tue Nov 02, 2010 2:30 pm
 
Forum: General Discussion
Topic: MAOI supplier
Replies: 1
Views: 1367
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