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Protease inhibitor

protease inhibitor

A drug that binds to and blocks hIV protease from working, thus preventing the production of new infectious viral articles.


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use of a bacterial protease inhibitor cocktail with mammalia

... some advice on Western Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore technical ...

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by rpwdoc
Tue Aug 26, 2014 10:08 pm
 
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 0
Views: 104

Amyloid beta (A4) precursor protein degradation products

... APP770. Compared with the other two isoforms, APP695 lacks the Kunitz protease inhibitor (KPI) region outside the cell. Neurons expressing APP695 subtypes in the cerebral ...

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by Anonymous
Tue Jun 12, 2012 3:11 am
 
Forum: General Discussion
Topic: Amyloid beta (A4) precursor protein degradation products
Replies: 1
Views: 6599

Re: Western Blot TroubleShooting

I harvest my samples using RIPA buffer + protease inhibitor on ice. I use a 6 well dish and I scrape the samples off on ice. I rotate the samples at 4C ...

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by mutant11
Mon Nov 21, 2011 3:11 am
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 3245

Western Blot TroubleShooting

... cell lines. Does anyone have any suggestions? -I use RIPA buffer with protease inhibitor to harvest the protein. I keep everything on ice. -I incubate in primary antibody ...

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by mutant11
Fri Nov 18, 2011 10:17 pm
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 3245

Removing MBP tag

... Here’re the facts: It’s a MBP-tag that should be removed. PreScission protease is used (I can’t use factor Xa because the size of Xa chains and ... well as on-column reaction. Further: reduced concentration of protease inhibitor, extended reaction time, added fresh protease after overnight incubation, ...

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by Papaver
Wed Aug 10, 2011 12:26 pm
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8703
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