Dictionary » P » Proofreading

Proofreading

proofreading

The property of certain polymerases e.g., dNA polymerase, to use their exonuclease activity to remove erroneously introduced bases and to replace them with the correct bases.


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Re: Why is ATP rather than GTP, CTP or TTP the energy currency?

... of translational machinery. GTP important for making reactions ` irreversible but accurate` GTP is important for fidelity of protein synthesis (proofreading after GTP hydrolysis) So the bottom line is – MUCH FASTER SPEED OF HYDROLYSIS is the reason ATP is chosen -evolutionary vise early on- ...

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by metingun2
Sun Oct 06, 2013 11:53 am
 
Forum: Molecular Biology
Topic: Why is ATP rather than GTP, CTP or TTP the energy currency?
Replies: 5
Views: 6040

How to clone a gene with lots of splicing intermediates?

... I have so far used varying primers on the cDNA of arabidopsis in order to get some fragments that I could then ligate together. I have used proofreading polymerase, Taq, phusion, abgene, and I have then tried (and failed) to isolate, purify and sequence the fragment. The sequence results ...

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by polkadotshorts
Sat Nov 24, 2012 6:49 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 3386

Re: Theories - Origin of Life

... once in every million nucleotides. This incredible copy fidelity is achieved by a sophisticated error-checking system involving base selection, proofreading and post replication repair. What has produced the incredible copy fidelity achieved by a sophisticated error-checking system involving ...

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by scottie
Tue Mar 20, 2012 2:22 pm
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 562
Views: 929123

Re: Theories - Origin of Life

... once in every million nucleotides. This incredible copy fidelity is achieved by a sophisticated error-checking system involving base selection, proofreading and postreplication repair. James Shapiro (evolutionary biologist) states Genome restructuring is a normal part of cellular life http://shapiro.bsd.uchicago.edu/Shapiro2009.AnnNYAcadSciMS.RevisitingCentral%20Dogma.pdf ...

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by scottie
Fri Mar 09, 2012 11:06 pm
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 562
Views: 929123

Re:

... of F+. This does not make any sense. What does correcting clone means? The purpose of replication is to multiply and spread genes.There are proofreading mechanisms, but mutation rates are highly variable even within a species, and in some case high mutation rates can be highly beneficial ...

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by ChesneMD
Thu Nov 17, 2011 4:13 am
 
Forum: Evolution
Topic: Viral-induced evolution
Replies: 6
Views: 5579
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