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Promoter insertion

promoter insertion

(Science: molecular biology) activation of a gene by the nearby integration of a virus. The long-terminal repeat acts as a promoter for the host gene. A form of insertional mutagenesis.


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LIC insertion for dual protein expression

... I will obtain then a linearized vector that, after treatment with T4 Pol+dATP I can anneal with a pETDuet LIC adaptor that will provide the T7 promoter, RBS, etc. for the expression of the second domain. As I am not a molecular biology expert I will appreciate the opinion of the forum about ...

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by cpmf752004
Mon May 23, 2011 7:21 pm
 
Forum: Molecular Biology
Topic: LIC insertion for dual protein expression
Replies: 0
Views: 848

What are methods to promote or suppress gene expression?

1) what is that? How do want to translate gene into promoter? What about simply expression of your gene of interest? 1) simply any ... know, whether it works in human. 5) in plants you could use simply T-DNA insertion :) but that's not specific. But some people belive, that Agrobacterium ...

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by JackBean
Thu Jul 29, 2010 7:22 am
 
Forum: Genetics
Topic: What are methods to promote or suppress gene expression?
Replies: 2
Views: 5222

Transcription inhibition

I am trying to develop a project where I can select bacteria for an insertion event (likely rare) in the genome. I know where the insertion will ... (no insertion) will therefore not express the resistance gene from the promoter upstream of the insertion site, while the modifications during the ...

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by canalon
Wed May 26, 2010 2:48 pm
 
Forum: Molecular Biology
Topic: Transcription inhibition
Replies: 2
Views: 3763

HELP ME PLEASE ......!!

... an RNA template C. DNA- ligase - joins segments of DNA D.transpose - insertion of DNA segments into DNA e. Spliceosome- removal of inrons 33. ... b. a repressible enzyme c. a restriction enzyme d. an operator e. a promoter 42. The DNA probe, GGCTTA, will hybridize with DNA containing – ...

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by BCH04
Mon Jun 18, 2007 4:15 pm
 
Forum: General Discussion
Topic: HELP ME PLEASE ......!!
Replies: 12
Views: 38574

HELP ME PLEASE ......!!

... an RNA template C. DNA- ligase - joins segments of DNA D.transpose - insertion of DNA segments into DNA e. Spliceosome- removal of inrons 33. ... b. a repressible enzyme c. a restriction enzyme d. an operator e. a promoter 42. The DNA probe, GGCTTA, will hybridize with DNA containing – ...

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by blcr11
Mon Jun 18, 2007 1:58 pm
 
Forum: General Discussion
Topic: HELP ME PLEASE ......!!
Replies: 12
Views: 38574
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