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Dictionary » P » Promoter PromoterDefinition noun, plural: promoters (chemistry) A substance that is capable of increasing the activity of a catalyst to increase the rate of reaction. (chemistry) A chemical that is supposed to promote carcinogenicity or mutagenicity. (genetics) A site in a DNA molecule at which RNA polymerase and transcription factors bind to initiate transcription of mRNA.
The site is actually specific DNA sequences that are recognized by transcription factors, which recruit RNA polymerase at the site. In the Operon Model, the promoter is the gene segment that serves as the initiation site where RNA polymerase binds to and initiates the transcription of certain genes. Conversely, in the presence of a repressor protein, this segment binds with the repressor. Because of this, the RNA polymerase cannot bind to it, resulting in the prevention of transcription of genes. And, this segment is now referred to as the operator gene.
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Results from our forumKnockout mice... retina was achieved by crossing Cdc42 floxed mice [29] with mice expressing cre recombinase under the control of the α element of the Pax6 promoter [30]. " From Mat'l & Methods: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553133/
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Re: Knockout mice... is a knock-down and not a knock-out. Watch out for the other meaning of knockdown: in addition to the meaning of having a genetically altered promoter to decrease expression of a protein, it is also used to describe inhibition at the RNA level using antisense oligos or siRNA.
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Reporter gene without fusionIf you mean the same promoter just separate (e.g. both under control of bGlu promoter), than the expression should be similar, because it should be on the same locus with the same strenght etc. However, if you mean (and I do not think ...
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Reporter gene without fusion... expression if it is fused with the foreign gene. But can we monitor the foreign gene expression if we place the reporter gene under a different promoter? If not, what is the use of placing a selectable marker and a reporter gene under the same construct (without a fusion) ? Thank you Regards :)
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Drosophila genesFrom which organisms is the following genetic elements derived? UAS promoter Options: bacteria drosophila yeast I think answer is drosophila Beta-galactosidase Options: bacteria drosophila yeast I think answer is bacteria Gal-4 Options: bacteria drosophila ...
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