Dictionary » P » Promoter

Promoter

Definition

noun, plural: promoters

(chemistry) A substance that is capable of increasing the activity of a catalyst to increase the rate of reaction.

(chemistry) A chemical that is supposed to promote carcinogenicity or mutagenicity.

(genetics) A site in a DNA molecule at which RNA polymerase and transcription factors bind to initiate transcription of mRNA.


Supplement

The site is actually specific DNA sequences that are recognized by transcription factors, which recruit RNA polymerase at the site.

In the Operon Model, the promoter is the gene segment that serves as the initiation site where RNA polymerase binds to and initiates the transcription of certain genes. Conversely, in the presence of a repressor protein, this segment binds with the repressor. Because of this, the RNA polymerase cannot bind to it, resulting in the prevention of transcription of genes. And, this segment is now referred to as the operator gene.


Word origin: promote » Middle English promoten, from Old French promoter, from Latin prōmovēre, prōmōt- : prō-, forward + -er.

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Sigma Factor Functions in Bacterial vs. Eukaryotes

PLEASE HELP! BIOCHEMISTRY HOMEWORK Sigma factor performs four important functions, each required before transcription can begin at a core promoter in bacteria. A) List the four functions. B) Name the transcription factors found in eukaryotes that provide these same four functions. PLEASE PLEASE ...

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by sabrina9
Sat Nov 02, 2013 9:49 pm
 
Forum: Molecular Biology
Topic: Sigma Factor Functions in Bacterial vs. Eukaryotes
Replies: 0
Views: 1094

The question about TATA sequence flexibility

Good day :) We know that: The TATA box is the sequence in DNA found in the promoter region of genes in archaea and eukaryotes that is responsible for recognizing the RNA-polymerase. http://en.wikipedia.org/wiki/TATA_box I read this article about TATA-Binding ...

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by Fantomas
Sat Aug 31, 2013 10:57 am
 
Forum: Molecular Biology
Topic: The question about TATA sequence flexibility
Replies: 1
Views: 1781

Transcrption factor-promoter (cis-element) interaction

... testing in plant or animal cells. I have been working with plant cells and the most common and easy way I guess is the reporter gene assay. the promoter in question is designed in a vector carrying the GUS reporter gene then you can measure GUS activity for instance...

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by bravebeaker
Thu Aug 22, 2013 3:31 am
 
Forum: Molecular Biology
Topic: Transcrption factor-promoter (cis-element) interaction
Replies: 3
Views: 1321

Transcrption factor-promoter (cis-element) interaction

I want to transfect my cells with a vector for promoter activity analaysis, I need to detect the binding effeciency of some transcription factors to the promoter, what technique should I use? I have read about ChIP but don't know how to design and ...

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by roniadam
Sun Aug 11, 2013 2:43 am
 
Forum: Molecular Biology
Topic: Transcrption factor-promoter (cis-element) interaction
Replies: 3
Views: 1321

cDNA cloning and Lentiviral transfection

... think that depends on the vector, which you've told us very little about.... The pWPT vector I used for lentiviral transfection has a bacterial promoter, origin of replication, and Ampicillin antibiotic resistance gene, to allow you to amplify it in bacteria. I don't know what your vector is, ...

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by BlahBlahBlah
Fri Jul 12, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 911
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