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Product surveillance, postmarketing

surveillance of drugs, devices, appliances, etc., for efficacy or adverse effects, after they have been released for general sale.


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Help: Confused with primers in a PCR of a vector?

... negative controls? Based on the few PCR reactions I tried drawing out myself (sorry, don't have an image), from my understanding, the amplified products I should get are: 1) T3 - coding region - T7 2) ??? It looks like it WON'T be a proper PCR reaction? Will we just get one massive chunk of ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 0
Views: 65

Please guide me about these restriction enzymes

I guess that depends rather on your plasmid https://www.neb.com/products/r0189-noti https://www.neb.com/products/r0131-nhei

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by JackBean
Tue Mar 11, 2014 2:20 pm
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 173

Increase in free energy in glycolysis

... other reactions are close to equilibrium (thus they can be used by gluconeogenesis as well) and only the actual concentrations of the substrates/products determine which way it will go.

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by JackBean
Thu Feb 06, 2014 10:42 am
 
Forum: Molecular Biology
Topic: Increase in free energy in glycolysis
Replies: 3
Views: 629

Action potentials of an earthworm going negative first

... looking at the action potentials of the earthworm. I anaesthatized the worms then hooked them up to a spiker box (http://backyardbrains.com/products/spikerbox) and stimulated them. This is an average result: http://i.stack.imgur.com/ezIEN.gif What I am confused about ...

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by IsDolan
Mon Jan 20, 2014 3:28 am
 
Forum: General Discussion
Topic: Action potentials of an earthworm going negative first
Replies: 2
Views: 373

A question regarding PCR

... will have, but it's not clear to me how to answer it. When the two DNA strand separate and have polymerases amplifying them, won't the initial products have different lengths since there is this little acatttg sequence before the first primer position? I mean, won't the product of the 5'-3' ...

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by x555
Sat Jan 18, 2014 11:05 am
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 454
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