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Procedures

procedure

A series of steps by which a desired result is accomplished.

Origin: L. Procedere, from pro = forward, cedere = move


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Why dialyse DMEM before protein purification (Ni-Affinity)?

... (50 mM NaH2PO4, 300 mM NaCl, pH 8). Afterwards Ni-NTA Agarose was added and finally these beads where put on a column. Some washing procedures followed (his-dialysis-buffer with either 10 mM imidazol or 50 mM imidazol) and in the end eluted (250 mM imidazol). I know that a dialysis ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 3
Views: 814

Re: Tools in Biology

Their goals are to make dishes with the most pleasing taste and the least process effort, so their hypotheses involve altering ingredients and procedures to optimize taste and processing. A hypothesis produces a recipe. They test the hypotheses by making the dish according to the recipe and assessing ...

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by jonmoulton
Wed Apr 11, 2012 10:37 pm
 
Forum: General Discussion
Topic: Tools in Biology
Replies: 3
Views: 2488

Re: Albinism

... to overcome this, but it is highly unlikely that anybody come to need therapy. To the best of my knowledge, there are no trustful medical procedures at present to revert albino condition in albino people that feel the need or want to. But I'm not a doctor nor a genetician. However, since ...

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by marquitosgm
Fri Mar 30, 2012 7:16 am
 
Forum: Human Biology
Topic: Albinism
Replies: 2
Views: 1011

Re:

... the examples you give on your website. I don't agree with you. The E. Coli experience and the Aids virus in a patient are the target of selective procedures: E. coli was able to survive after 30.000 generations in citric acid and the Aids virus in people has to fight the antivirus drugs the patients ...

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by christianstrategies
Mon Feb 27, 2012 8:31 pm
 
Forum: Evolution
Topic: Any SOLID arguments against evolution?
Replies: 309
Views: 430337

Chitosan Extraction

... research that will involve the extraction of chitosan from crab shells. I need help with the extraction of chitosan from them. I found some procedures online that were used in past research studies, but they're just an overview. I need the exact procedure, step-by-step. Which chemicals to ...

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by kevin81797
Mon Feb 20, 2012 11:59 pm
 
Forum: Zoology Discussion
Topic: Chitosan Extraction
Replies: 1
Views: 968
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