Probes

... it is always possible, though unlikely, that you have a disease-causing mutation that is not previously known and thus there are no suitable probes and primers for it at all. Furthermore, to get any useful information (especially if doing the testing yourself), you need extensive knowledge ...

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... I see people do in situ on slides, but I work with 96-well plates. I use the Perkin Elmer TSA plus kit (cy3/Fluorescein) for two colors. Two mRNA probes (one is Digoxigenin-labeled the other is Fluorescein labeled) were added to the sample and hybridized at 65C overnight. I use anti-Digoxigenin-POD ...

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F1 progeny is in the left, The BB is a testcross. M1 and M2 are probes that attached to DNA fragments which was cut by restriction enzyme. The bottom table shows the gel-electropohresis analyse. The table gametes shows the 4 possible gametes between F1 progeny(red ...

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elynn: in such case it would be probably best to prepare slightly degenerated probes for whole length and do some screening of libraries.

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... Time PCR, I am using this method to detect the expression levels (upregulated or downregulated) of mRNA in different samples, is it better to use probes or SYBR Green? Why? This is a stupid question, but when making primers, how does one decide whether to use the sequence from a mouse vs human ...

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