Probes

... cell biologist, for example. A simple blood test is required, and the lymphocytes are stained and analysed visually. An X and Y specific stains / probes indicate whether there is an extra set of chromosomes or other such "apparent" anomalities in the genome. This does not reveal genetic ...

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... techniques are really vague :/ I mean, i can understand how you can identify a specific gene or RFLP using hybridization, or RT-PCR with specific probes - but how do you find a brand new gene involved with filiment formation that has no homologue? I think it's something to do with RNA that's made ...

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Hello，Did somebody use EnzChek Lysozyme Assay Kit(invitrogen,molecular probes)? I meet some difficulty in determining lysozyme activity using the EnzChek Lysozyme Assay Kit(invitrogen,molecular probes). the fluorescence of samples is very low and out of standard ...

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For the hybridization I would say the specificity (100% match with target sequence) and amount of the probes (should be in excess..) then maybe high washing temperature and low salt concentration to wash away the unspecific hybridization.. hope it'll help..

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Hi, I am having problems with making DIG RNA probes (Roche). I get no bands when running a gel after purification other than the weak band of the linearised plasmid. I am using T7 and SP6 polymerase. I seem to have narrowed down the problem to ...

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