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Probes

probe syringe

A syringe with an olive-shaped tip, used in treatment of diseases of the lacrimal passages.


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hybridization probe

I will use lightcycler 2.0. to detect and SNP with hybridization probes. I designed the probes but I couldn't choose fluorescent dyes, and don't know the criterias. Could you please help me...

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by FDHeLiX
Mon Sep 16, 2013 3:33 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 1499

Another molecular question

... by more than one triplet. But if you manage to select piece where are mostly AAs coded by only one or two codons, you could prepare degenerated probes and use them for the hybridization. However, that will not work for hemoglobin in erythrocytes.

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by JackBean
Wed Aug 21, 2013 1:37 pm
 
Forum: Molecular Biology
Topic: Another molecular question
Replies: 3
Views: 1134

Genetic testing: reliability, accuracy, experience?

... it is always possible, though unlikely, that you have a disease-causing mutation that is not previously known and thus there are no suitable probes and primers for it at all. Furthermore, to get any useful information (especially if doing the testing yourself), you need extensive knowledge ...

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by biohazard
Mon Apr 15, 2013 8:55 am
 
Forum: Genetics
Topic: Genetic testing: reliability, accuracy, experience?
Replies: 3
Views: 3990

Two-color in situ help

... I see people do in situ on slides, but I work with 96-well plates. I use the Perkin Elmer TSA plus kit (cy3/Fluorescein) for two colors. Two mRNA probes (one is Digoxigenin-labeled the other is Fluorescein labeled) were added to the sample and hybridized at 65C overnight. I use anti-Digoxigenin-POD ...

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by vastgenome
Thu Feb 14, 2013 5:00 am
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 2211

Re: RFLPs

F1 progeny is in the left, The BB is a testcross. M1 and M2 are probes that attached to DNA fragments which was cut by restriction enzyme. The bottom table shows the gel-electropohresis analyse. The table gametes shows the 4 possible gametes between F1 progeny(red ...

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by dreamtim
Wed Oct 10, 2012 8:56 pm
 
Forum: Genetics
Topic: RFLPs
Replies: 3
Views: 1737
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