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Probe-pointed

probe-pointed

(Science: surgery) Having a blunt or button-shaped extremity; said of cutting instruments.


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FISH and Taqman probes

I want to quantify bacteria , and this bacteria has been detected by using FISH probe(targets 16S). Can I use this FISH probe as my TaqMan probe and design the forward and reverse primers on the two sides of it?

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by 2444256703
Tue Mar 03, 2015 6:28 am
 
Forum: General Discussion
Topic: FISH and Taqman probes
Replies: 0
Views: 121

Taqman probe design using FISH probe (need help)

Hi: Although they are both called "probes", FISH and Taqman probes have different characteristics. Probes for FISH need to be at least 1 kb long, to hybridize and "highlight" the target chromosomal DNA (metaphase chromosomes ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 571

Taqman probe design using FISH probe (need help)

... I need your help please. I want to quantify bacteria by using RT-PCR (Taqman), this bacteria has been detected (in previous study) by using FISH probe (targets 16S). Can I use this FISH probe (from the paper) as my TaqMan probe and design the forward and reverse primers on the two sides of it? ...

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by biomart
Wed Feb 04, 2015 7:10 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 571

Souther blot and restriction mapping

... through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised to and I am struggling to find the correct approach. The parting words from lecturer were 'It's just a matter of logic, consider ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 1
Views: 1604

Measuring ROS with DCFH probe

What is the probe supposed to detect? The ROS directly?

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by JackBean
Wed Mar 05, 2014 10:20 am
 
Forum: Molecular Biology
Topic: Measuring ROS with DCFH probe
Replies: 1
Views: 1848
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