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Probe

probe

(Science: molecular biology) general term for a piece of dNA or rNA corresponding to a gene or sequence of interest, that has been labelled either radioactively or with some other detectable molecule, such as biotin, digoxygenin or fluorescein. As stretches of DNA or RNA with complementary sequences will (hybridise), a probe will label viral plaques, bacterial colonies or bands on a gel that contain the gene of interest.

See: northern blots, southern blots.


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Measuring ROS with DCFH probe

What is the probe supposed to detect? The ROS directly?

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by JackBean
Wed Mar 05, 2014 10:20 am
 
Forum: Molecular Biology
Topic: Measuring ROS with DCFH probe
Replies: 1
Views: 499

Would an asteroid simply just pass through Jupiter

... on it that as you descend towards the core it will form a liquid, and eventually even a metallic substance! The Galileo spacecraft dropped a probe into the atmosphere and it burned up after about 150 miles - that's not too far given the size of the planet! An additional thing to consider ...

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by Babybel56
Fri Feb 28, 2014 8:38 am
 
Forum: General Discussion
Topic: Would an asteroid simply just pass through Jupiter
Replies: 4
Views: 291

Would an asteroid simply just pass through Jupiter

... exerted on this hydrogen will make it turn into a liquid, and eventually even a metallic layer towards the core. The Galileo spacecraft dropped a probe into the atmosphere and it made it about 150miles before burning up - that's not very far in terms of Jupiter's size! It may be a gas giant, but ...

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by Babybel56
Fri Feb 28, 2014 8:35 am
 
Forum: General Discussion
Topic: Would an asteroid simply just pass through Jupiter
Replies: 4
Views: 291

Re: qPCR vs Northern Blot

... weeks qPCR). @jonmoulton - you're rigth first - in Northern blot I had silencing, in qPCR i didn't. As far as your question is concerned - my NB probe covers whole length of mRNA - all exons (it's a short one ~2 kbp total). In case of my PCR primers - FORWARD primer binds in one exon in the middle ...

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by Nevthonien
Tue Nov 12, 2013 2:47 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1270

Re: qPCR vs Northern Blot

I assume you are assaying aliquots of the same RNA samples by the two methods. Does your Northern probe bind between the binding sites of your PCR primers? If not, it is conceivable that a change in splicing in the presence of the shRNA causes your Northern target to be ...

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by jonmoulton
Thu Nov 07, 2013 10:12 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1270
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