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Probe

probe

(Science: molecular biology) general term for a piece of dNA or rNA corresponding to a gene or sequence of interest, that has been labelled either radioactively or with some other detectable molecule, such as biotin, digoxygenin or fluorescein. As stretches of DNA or RNA with complementary sequences will (hybridise), a probe will label viral plaques, bacterial colonies or bands on a gel that contain the gene of interest.

See: northern blots, southern blots.


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FISH and Taqman probes

I want to quantify bacteria , and this bacteria has been detected by using FISH probe(targets 16S). Can I use this FISH probe as my TaqMan probe and design the forward and reverse primers on the two sides of it?

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by 2444256703
Tue Mar 03, 2015 6:28 am
 
Forum: General Discussion
Topic: FISH and Taqman probes
Replies: 0
Views: 103

Taqman probe design using FISH probe (need help)

Hi: Although they are both called "probes", FISH and Taqman probes have different characteristics. Probes for FISH need to be at least 1 kb long, to hybridize and "highlight" the target chromosomal DNA (metaphase chromosomes ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 528

Taqman probe design using FISH probe (need help)

... I need your help please. I want to quantify bacteria by using RT-PCR (Taqman), this bacteria has been detected (in previous study) by using FISH probe (targets 16S). Can I use this FISH probe (from the paper) as my TaqMan probe and design the forward and reverse primers on the two sides of it? ...

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by biomart
Wed Feb 04, 2015 7:10 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 528

Souther blot and restriction mapping

... through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised to and I am struggling to find the correct approach. The parting words from lecturer were 'It's just a matter of logic, consider ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 1
Views: 1577

Measuring ROS with DCFH probe

What is the probe supposed to detect? The ROS directly?

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by JackBean
Wed Mar 05, 2014 10:20 am
 
Forum: Molecular Biology
Topic: Measuring ROS with DCFH probe
Replies: 1
Views: 1835
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