Dictionary » P » Primers

Primers

Primers: Syntheic nucleotide strands inserted for pairing with parent template of DNA; usually it is installed at the opposite ends of the strand, and zipped back together by dna polymerase. The primary use for primers is building recombintant dna. Genetic Engineers use primers to combine different organisms dna and introduce alternate forms. Primer is one of main insert in polymerase chain reaction, which speed up replication process.


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An interesting PCR smearing...I dont know what to do :(

Just a stupid idea, but if you digest your DNA with some RE cutting between your primers, do you get rid of the smear?

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by JackBean
Sat Jun 28, 2014 6:08 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 6530

Re: An interesting PCR smearing...I dont know what to do :(

hello all , Even i have a similar problem. I am using 3 different primers to amplify 3 different exons of a gene . 1 of the exon is amplifying very well ( a single bright band ) but the other two exon amplification is not coming properly.and this has occured ...

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by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 6530

Help: Confused with primers in a PCR of a vector?

... gene is right next to T7: T7-gene. If your gene is inserted into the vector so that the beginning of the gene is next to T3: Nothing since both primers bind in the same direction. 3) if your gene is inserted into the vector so the beginning of the gene is right next to T7: Nothing since both ...

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by poobear
Fri Apr 11, 2014 9:58 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2430

Help: Confused with primers in a PCR of a vector?

... our gene of interest is located), of which is "flanked" by the T3 and T7 promoters. The thing that throws me off is just where these primers will bind to, and what the subsequent product will be. The one that really throws me off is number 2, because it seems like we might not get ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2430

Annealing Temparature

The primers obviously contain added restriction sites and the matching part is quite short. I would anneal the first cycle at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C ...

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 1461
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