Dictionary » P » Primers

Primers

Primers: Syntheic nucleotide strands inserted for pairing with parent template of DNA; usually it is installed at the opposite ends of the strand, and zipped back together by dna polymerase. The primary use for primers is building recombintant dna. Genetic Engineers use primers to combine different organisms dna and introduce alternate forms. Primer is one of main insert in polymerase chain reaction, which speed up replication process.


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Help: Confused with primers in a PCR of a vector?

... our gene of interest is located), of which is "flanked" by the T3 and T7 promoters. The thing that throws me off is just where these primers will bind to, and what the subsequent product will be. The one that really throws me off is number 2, because it seems like we might not get ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 0
Views: 65

Annealing Temparature

The primers obviously contain added restriction sites and the matching part is quite short. I would anneal the first cycle at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C ...

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 350

Totipotent Stem Cell From Molecular Components

... Multiply by 100 to get the amount of protein Q4 I have heard of amplicons used in PCR. Do I really need one to copy a whole gene or just primers, DNA, and enzymes?

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by caters
Sun Feb 02, 2014 10:58 pm
 
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 0
Views: 573

mRNA Search for RT-PCR (U to T)

Because first you synthesise 1st strand and then use the primers to amplify your gene.

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by JackBean
Fri Jan 24, 2014 12:58 pm
 
Forum: Molecular Biology
Topic: mRNA Search for RT-PCR (U to T)
Replies: 8
Views: 4241

A question regarding PCR

... cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca cacacattcg acggttgcag GCAAATACTG CCCTGTGGGG-3’ The places where primers should attach are written in capitals. My question is how many base pairs the amplified product will have, but it's not clear to me how to answer ...

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by x555
Sat Jan 18, 2014 11:05 am
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 455
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