Dictionary » P » Primers

Primers

Primers: Syntheic nucleotide strands inserted for pairing with parent template of DNA; usually it is installed at the opposite ends of the strand, and zipped back together by dna polymerase. The primary use for primers is building recombintant dna. Genetic Engineers use primers to combine different organisms dna and introduce alternate forms. Primer is one of main insert in polymerase chain reaction, which speed up replication process.


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FISH and Taqman probes

... this bacteria has been detected by using FISH probe(targets 16S). Can I use this FISH probe as my TaqMan probe and design the forward and reverse primers on the two sides of it?

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by 2444256703
Tue Mar 03, 2015 6:28 am
 
Forum: General Discussion
Topic: FISH and Taqman probes
Replies: 0
Views: 110

Taqman probe design using FISH probe (need help)

... releasing the fluorescence (quenched in intact probe) that will be measured to quantify the starting level of template. Therefore, you can design primers and probes so that they will fall within the sequence recognized by the FISH probe. you can easily find instructions for designing good quality ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 543

Taqman probe design using FISH probe (need help)

... study) by using FISH probe (targets 16S). Can I use this FISH probe (from the paper) as my TaqMan probe and design the forward and reverse primers on the two sides of it? If it’s possible how can I do it? Thank you for your help

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by biomart
Wed Feb 04, 2015 7:10 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 543

principle of error-prone PCR???

... Mn stabilizes double stranded DNA and from practical PCR standpoint - excessive magnesium allows for annealing and elongation of mismatched primers.

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by Cat
Sun Oct 12, 2014 7:39 pm
 
Forum: Molecular Biology
Topic: principle of error-prone PCR???
Replies: 5
Views: 2484

An interesting PCR smearing...I dont know what to do :(

Just a stupid idea, but if you digest your DNA with some RE cutting between your primers, do you get rid of the smear?

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by JackBean
Sat Jun 28, 2014 6:08 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 8779
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