Primer

Because if the amino acids are identical, the DNA sequences are probably also very similar (but does not have to be identical!), thus primer annealing to this region may be used for several species. It can be used either as unspecific primer for known species or it can be used even for so ...

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Why would the identical amino acids region of certain species be used as a basis for a primer design? I know they share homology and are very conserved but why is it used as a primer?

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"what i don't know is why the exact same primers that target the same location end up a different areas." I don't think that the same primers that target the same location do end up in different areas. The difference in the masses of the ...

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I realise that each of the 12 primer pairs bind to a different region of human genome; the VNTR's within each region, and that is how 12 distinct bands can be detected after PCR. But what i don't know is why the exact same primers that target ...

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Now I see your point, but in my case I use the primer mix that comes with the RT kit by Takara..

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