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Primer

primer

short pre-existing polynucleotide chain towhich new deoxyribonucleotides can be added by dNA polymerase.


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No "beginning-replication problem" on leading strand?

... at the origins and from those a continuous strand is produced until meeting replicating fork from another direction or end of the strand. The primer is removed by Okazaki fragment going in the other direction.

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by JackBean
Tue May 13, 2014 4:55 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 897

No "beginning-replication problem" on leading strand?

... read why we have telomers, the answer is always so that we can replicate the end of the lagging strand. This is because the extreme end have a RNA primer to make the last Okazaki fragment, and after the removal of this primer it needs to somehow be replaced with DNA. I have the latest version of ...

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by poobear
Fri Apr 11, 2014 9:40 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 897

DNA testing times

... to see whether they are the same species, AND if one of the organisms is known to be a particular species AND if the lab has a proven PCR primer set specific for that species and has previously run some assays using that primer set, then the analysis is fairly straightforward - just see ...

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by jonmoulton
Fri Apr 11, 2014 2:47 pm
 
Forum: Genetics
Topic: DNA testing times
Replies: 2
Views: 1210

Help: Confused with primers in a PCR of a vector?

... something I do not really understand. We used the pBS-gene as our template. We carried out four reactions, which are as followed: 1) T7 (forward primer); T3 (reverse primer) 2) T7 (forward primer); coding reverse (reverse primer) 3) T7 (forward primer); coding forward (reverse primer) 4) T3 (forward ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2687

Re: Annealing Temparature

Primer_F: 5'-GCTCTAGAGTGATAGTGAGCATGG-3' Primer_R: 5'-GCGATATCCTACACCCAAGCTGC-3' CORRECTED PRIMER SEQUENCE. THANKS ALL. I WILL TRY SUGGESTED EXP. CONDITION. THANKS "vladCH".

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by Helics
Sun Feb 23, 2014 4:59 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 1579
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