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Primer

primer

short pre-existing polynucleotide chain towhich new deoxyribonucleotides can be added by dNA polymerase.


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Why would the identical amino acids region of certain specie

Because if the amino acids are identical, the DNA sequences are probably also very similar (but does not have to be identical!), thus primer annealing to this region may be used for several species. It can be used either as unspecific primer for known species or it can be used even for so ...

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by JackBean
Mon Feb 18, 2013 1:06 pm
 
Forum: Genetics
Topic: Why would the identical amino acids region of certain specie
Replies: 2
Views: 179

Why would the identical amino acids region of certain specie

Why would the identical amino acids region of certain species be used as a basis for a primer design? I know they share homology and are very conserved but why is it used as a primer?

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by TToe
Mon Feb 18, 2013 10:37 am
 
Forum: Genetics
Topic: Why would the identical amino acids region of certain specie
Replies: 2
Views: 179

Re: PCR

"what i don't know is why the exact same primers that target the same location end up a different areas." I don't think that the same primers that target the same location do end up in different areas. The difference in the masses of the ...

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by jonmoulton
Tue Jan 29, 2013 4:16 pm
 
Forum: Genetics
Topic: PCR
Replies: 2
Views: 502

PCR

I realise that each of the 12 primer pairs bind to a different region of human genome; the VNTR's within each region, and that is how 12 distinct bands can be detected after PCR. But what i don't know is why the exact same primers that target ...

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by Julian92
Tue Jan 29, 2013 3:10 pm
 
Forum: Genetics
Topic: PCR
Replies: 2
Views: 502

RT-PCR

Now I see your point, but in my case I use the primer mix that comes with the RT kit by Takara..

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by bravebeaker
Tue Jan 29, 2013 1:19 am
 
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 3264
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