Dictionary » P » Primer

Primer

primer

short pre-existing polynucleotide chain towhich new deoxyribonucleotides can be added by dNA polymerase.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


amplification in no RT control

... and my NTC is not amplifying. This suggests to me that I am not getting dimers and my reagents are 'good' I have tried cDNA at 10ng and 5ng and primer conc. at both 200uM and 400uM. I'm kind of out of ideas now...more/longer DNase digestion? Or does this sound more like an optimisation issue? ...

See entire post
by biomart
Fri Mar 20, 2015 1:58 am
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 0
Views: 39

No "beginning-replication problem" on leading strand?

... at the origins and from those a continuous strand is produced until meeting replicating fork from another direction or end of the strand. The primer is removed by Okazaki fragment going in the other direction.

See entire post
by JackBean
Tue May 13, 2014 4:55 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 1905

No "beginning-replication problem" on leading strand?

... read why we have telomers, the answer is always so that we can replicate the end of the lagging strand. This is because the extreme end have a RNA primer to make the last Okazaki fragment, and after the removal of this primer it needs to somehow be replaced with DNA. I have the latest version of ...

See entire post
by poobear
Fri Apr 11, 2014 9:40 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 1905

DNA testing times

... to see whether they are the same species, AND if one of the organisms is known to be a particular species AND if the lab has a proven PCR primer set specific for that species and has previously run some assays using that primer set, then the analysis is fairly straightforward - just see ...

See entire post
by jonmoulton
Fri Apr 11, 2014 2:47 pm
 
Forum: Genetics
Topic: DNA testing times
Replies: 2
Views: 2535

Help: Confused with primers in a PCR of a vector?

... something I do not really understand. We used the pBS-gene as our template. We carried out four reactions, which are as followed: 1) T7 (forward primer); T3 (reverse primer) 2) T7 (forward primer); coding reverse (reverse primer) 3) T7 (forward primer); coding forward (reverse primer) 4) T3 (forward ...

See entire post
by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 4613
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 27,953 times. 
What links here | Related changes | Permanent link