Primer

Have you checked for contamination in your reagents by using the kits on a previously tested primer that you know gives the right product size? My lab recently had an issue with contamination in our SuperScript mix that was giving us unwanted banding in our gels and it ...

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Hi I designed a specific primers and blast it to amplify my gene. this primer amplifies a gDNA of the gene. I used dreamtaq kit and its protocol but unfortunately the expected product (2054 bp) was not there and what I got is several ...

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Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination ...

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There's a primer designer that's highlighted in this blog post: http://thebioblog.com/2009/08/5-great-online-tools-for-biologists/

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I need immediate help!! How to PCR primer designing? I know primer design based on comparison of sequences of specific gene from GenBank. Conserved regions were identified by using software Clustal X/W. Than primer A was designed based on ...

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