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Primase

primase

(Science: enzyme) The enzyme that polymerises nucleotide triphosphates to form oligoribonucleotides in a 5' to 3' direction. The enzyme synthesises the rNA for RNA dNA sequences that later become okazaki fragments and also rNA primers for some types of phage using an sDNA template.


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How does a plasmid work?

... go into a little more detail about how the replication works? Or link me to a website? Is it still the same with helicase unwinding the DNA and primase coming in and etc. like in regular DNA replication?

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by WizardofScience
Sun Dec 30, 2012 2:06 pm
 
Forum: Molecular Biology
Topic: How does a plasmid work?
Replies: 2
Views: 1917

Topoisomerase DNA Replication :D

Will the Okazaki fragment in Topoisomerase DNA replication always be between the primase and the ligase protein complexes on the lagging strand? Are there any exceptions to this?? Thanks very much, Billy :D

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by billyfisher100
Wed Mar 28, 2012 6:37 pm
 
Forum: Cell Biology
Topic: Topoisomerase DNA Replication :D
Replies: 2
Views: 2336

Re: DNA Replication Lagging Strand

Oh My! :D You are right! Primase is actually RNA polymerase . So the order should be like that; ⋅ RNA polymerase (add primer) ⋅ DNA polymerase (replicate) ⋅ RNAse (remove primer) ⋅ DNA polymerase ...

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by BeyazRus
Tue Sep 21, 2010 10:25 pm
 
Forum: Genetics
Topic: DNA Replication Lagging Strand
Replies: 6
Views: 5798

DNA Replication Lagging Strand

Yes but what kind of family does the primase belong to? Considering it is making RNA... And considering that the question is about the type of activity rather than the exact enzyme, the offered answer makes sense.

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by canalon
Tue Sep 21, 2010 7:24 pm
 
Forum: Genetics
Topic: DNA Replication Lagging Strand
Replies: 6
Views: 5798

Re: DNA Replication Lagging Strand

Primers made by primase enzyme. However, it is not found in question. After helicase enzyme seperate two strands, Primase adds primers. After then, the order is what firecloak said. DNA polymerase RNAse (remove primer) DNA polymerase ...

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by BeyazRus
Tue Sep 21, 2010 2:33 pm
 
Forum: Genetics
Topic: DNA Replication Lagging Strand
Replies: 6
Views: 5798
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