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Primase

primase

(Science: enzyme) The enzyme that polymerises nucleotide triphosphates to form oligoribonucleotides in a 5' to 3' direction. The enzyme synthesises the rNA for RNA dNA sequences that later become okazaki fragments and also rNA primers for some types of phage using an sDNA template.


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Purpose of a primase/polymerase localized to the cytosol?

Hi, I recently read a paper, "PrimPol, an Archaic Primase/Polymerase Operating in Human Cells" by Garcia-Gomez et al. This polymerase was largely localized to the cytosol - I'm wondering if anyone has ideas as to why that would be the case ...

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by rhardison
Fri Oct 03, 2014 8:51 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 0
Views: 20

How does a plasmid work?

... go into a little more detail about how the replication works? Or link me to a website? Is it still the same with helicase unwinding the DNA and primase coming in and etc. like in regular DNA replication?

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by WizardofScience
Sun Dec 30, 2012 2:06 pm
 
Forum: Molecular Biology
Topic: How does a plasmid work?
Replies: 2
Views: 1969

Topoisomerase DNA Replication :D

Will the Okazaki fragment in Topoisomerase DNA replication always be between the primase and the ligase protein complexes on the lagging strand? Are there any exceptions to this?? Thanks very much, Billy :D

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by billyfisher100
Wed Mar 28, 2012 6:37 pm
 
Forum: Cell Biology
Topic: Topoisomerase DNA Replication :D
Replies: 2
Views: 2395

Re: DNA Replication Lagging Strand

Oh My! :D You are right! Primase is actually RNA polymerase . So the order should be like that; ⋅ RNA polymerase (add primer) ⋅ DNA polymerase (replicate) ⋅ RNAse (remove primer) ⋅ DNA polymerase ...

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by BeyazRus
Tue Sep 21, 2010 10:25 pm
 
Forum: Genetics
Topic: DNA Replication Lagging Strand
Replies: 6
Views: 5895

DNA Replication Lagging Strand

Yes but what kind of family does the primase belong to? Considering it is making RNA... And considering that the question is about the type of activity rather than the exact enzyme, the offered answer makes sense.

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by canalon
Tue Sep 21, 2010 7:24 pm
 
Forum: Genetics
Topic: DNA Replication Lagging Strand
Replies: 6
Views: 5895
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