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Pribnow box

Pribnow box --> promoter

(Science: molecular biology) A region of dNA to which rNA polymerase binds before initiating the transcription of DNA into rNA.

The nucleotide at which transcription starts is designated _1 and nucleotides are numbered from this with negative numbers indicating upstream nucleotides and positive downstream nucleotides.

most bacterial promoters contain two consensus sequences that seem to be essential for the binding of the polymerase. The first, the pribnow box, is at about 10 and has the consensus sequence 5' TATAAT 3'. The second, the 35 sequence, is centred about 35 and has the consensus sequence 5' TTGACA 3'.

most factors that regulate gene transcription do so by binding at or near the promoter and affecting the initiation of transcription. Much less is known about eukaryote promoters, each of the three rNA polymerases has a different promoter.

rNA polymerase I recognises a single promoter for the precursor of rRNA.

rNA polymerase II, that transcribes all genes coding for polypeptides, recognises many thousands of promoters. most have the Goldberg Hogness or tATA box that is centred around position 25 and has the consensus sequence 5' TATAAAA 3'. Several promoters have a cAAT box around 90 with the consensus sequence 5' GGCCAATCT 3'. There is increasing evidence that all promoters for housekeeping genes contain multiple copies of a gC rich element that includes the sequence 5' GGGC 731 GG 3'. Transcription by polymerase II is also affected by more distant elements known as enhancers.

rNA polymerase III synthesises 5s ribosomal RNA, all tRNAs and a number of small rNAs. The promoter for RNA polymerase III is located within the gene either as a single sequence, as in the 5s RNA gene or as two blocks, as in all tRNA genes.


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RNA Transcription and Sigma Factor

... with the RNA polymerase. Region 2.4 recognizes and binds to the Pribnow box. Region 4.2 recognizes and binds to the -35 promoter site.

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by aptitude
Thu Oct 13, 2011 11:01 pm
 
Forum: Molecular Biology
Topic: RNA Transcription and Sigma Factor
Replies: 1
Views: 2541

Can anyone help with this RNA question

edit - the pribnow box is nucleotides 198-203

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by georgina009
Mon Mar 17, 2008 5:35 am
 
Forum: Molecular Biology
Topic: Can anyone help with this RNA question
Replies: 5
Views: 4478

Re: Can anyone help with this RNA question

... nucleotides 86-91 and the start codon is 226. Then what i think is the pribnow box is CATAAT which is nucleotides 198-123. So i guess what I really what to know is: is the ...

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by georgina009
Mon Mar 17, 2008 5:34 am
 
Forum: Molecular Biology
Topic: Can anyone help with this RNA question
Replies: 5
Views: 4478

Re: Can anyone help with this RNA question

... are generally composed of two very roughly conserved features. The Pribnow Box sits 5-8 bases upstream of the transcriptional start site (almost always an A or G), while ...

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by blcr11
Wed Mar 12, 2008 3:35 pm
 
Forum: Molecular Biology
Topic: Can anyone help with this RNA question
Replies: 5
Views: 4478

Can anyone help with this RNA question

... a strand of E. coli mRna and I can't figure out where the promoter and pribnow box are. It looks like this: TACTCCACAT AATTGTCCTT CTTTTTAGAG GAAAAATG So it ends in the start ...

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by georgina009
Wed Mar 12, 2008 12:04 am
 
Forum: Molecular Biology
Topic: Can anyone help with this RNA question
Replies: 5
Views: 4478
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