Pribnow

edit - the pribnow box is nucleotides 198-203

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Sorry I only wrote a short bit. I found an area of TTGACA but it is nucleotides 86-91 and the start codon is 226. Then what i think is the pribnow box is CATAAT which is nucleotides 198-123. So i guess what I really what to know is: is the TTGACA got anything to do with it so that if it were ...

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... not the same thing as the translational start site. Bacterial promoter regions are generally composed of two very roughly conserved features. The Pribnow Box sits 5-8 bases upstream of the transcriptional start site (almost always an A or G), while the +35 region sits roughly 30-40 bases upstream ...

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i was given a strand of E. coli mRna and I can't figure out where the promoter and pribnow box are. It looks like this: TACTCCACAT AATTGTCCTT CTTTTTAGAG GAAAAATG So it ends in the start codon and the Shine dalgarno sequence is GAGGA (I think, correct me if I'm wrong) ...

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... And for good measure, here is another question: What happens if a promotor motif is incorporated within an operon? Surely this can happen, as the pribnow box etc. are not that specific. How would the holoenzyme know not to bind in the middle of 'another' operon? I look forward to your replies. ...

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