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Pribnow

Pribnow

David, 20th-century U.S. Molecular biologist.

See: pribnow box.


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promoter mutations effects

... I find promoter regions on this sequence and question that: (- 35 REGİON: TTGACA, -10 REGİON: TCCAAT) Please answer mutation questions on Pribnow sequences 1-change last T residue of pribnow sequence to A, 2-change first A residue of pribnow sequence to T residue, Please answer mutation ...

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by aves
Sun Nov 20, 2011 5:52 pm
 
Forum: Molecular Biology
Topic: promoter mutations effects
Replies: 2
Views: 1285

RNA Transcription and Sigma Factor

... in ensuring the sigma factor will only bind the promoter when it is complexed with the RNA polymerase. Region 2.4 recognizes and binds to the Pribnow box. Region 4.2 recognizes and binds to the -35 promoter site.

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by aptitude
Thu Oct 13, 2011 11:01 pm
 
Forum: Molecular Biology
Topic: RNA Transcription and Sigma Factor
Replies: 1
Views: 2323

Can anyone help with this RNA question

edit - the pribnow box is nucleotides 198-203

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by georgina009
Mon Mar 17, 2008 5:35 am
 
Forum: Molecular Biology
Topic: Can anyone help with this RNA question
Replies: 5
Views: 4045

Re: Can anyone help with this RNA question

Sorry I only wrote a short bit. I found an area of TTGACA but it is nucleotides 86-91 and the start codon is 226. Then what i think is the pribnow box is CATAAT which is nucleotides 198-123. So i guess what I really what to know is: is the TTGACA got anything to do with it so that if it were ...

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by georgina009
Mon Mar 17, 2008 5:34 am
 
Forum: Molecular Biology
Topic: Can anyone help with this RNA question
Replies: 5
Views: 4045

Re: Can anyone help with this RNA question

... not the same thing as the translational start site. Bacterial promoter regions are generally composed of two very roughly conserved features. The Pribnow Box sits 5-8 bases upstream of the transcriptional start site (almost always an A or G), while the +35 region sits roughly 30-40 bases upstream ...

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by blcr11
Wed Mar 12, 2008 3:35 pm
 
Forum: Molecular Biology
Topic: Can anyone help with this RNA question
Replies: 5
Views: 4045
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