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Preparation

preparation

1. (Science: pharmacology) A medicine made ready for use.

2. (Science: anatomy, pathology) A specimen made ready and preserved for study.

Origin: L. Praeparatio


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TAE Buffer preparation

Please how do I prepare 10 M tae buffer containing 2M tris, 0.5M edta, 17.4M acetic acid and adjusted to pH 8.0 with NaOH .

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by dunamis
Thu Feb 26, 2015 6:05 pm
 
Forum: Molecular Biology
Topic: TAE Buffer preparation
Replies: 0
Views: 299

Plasmid yield/concentration

... yield plasmid" means that host cells make a lot of copies per cell and that usually results in high plasmid concentration in the final DNA preparation. Alternatively, larger volume of stating material to smaller volume elution can be used to concentrate low yield plasmid prep...

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by Cat
Sat Sep 20, 2014 4:18 pm
 
Forum: Molecular Biology
Topic: Plasmid yield/concentration
Replies: 3
Views: 2558

DNA testing times

I'm not doing this stuff these days, so you should find a better source for confirmation. In general the process involves: (1) sample preparation (2) running an assay (3) analysis The time required will depend on what sort of information you need and therefore what assay you would need to ...

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by jonmoulton
Fri Apr 11, 2014 2:47 pm
 
Forum: Genetics
Topic: DNA testing times
Replies: 2
Views: 2536

preparation of Carboxymethyllysine (CML)

Hi, Preparation of CML as a major Advanced Glycation Endproducts in the lab is too critical, we usually use BSA and glyoxalic acid, and we add NaCNBH3 to the mixture. Does anyone know why we add NaCNBH3 to prepare CML? Thanks.

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by ALJOHI
Thu Jan 02, 2014 3:41 pm
 
Forum: Cell Biology
Topic: preparation of Carboxymethyllysine (CML)
Replies: 1
Views: 1859

Carnoy's fixation problem

Hi everyone, What should be the original concentration of ethanol or methanol used in the preparation of Carnoy's fixative solution? I know it is alcohol: glacial acetic acid( 3:1). But what is the original concentration of the alcohol? Thank you very much :)

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by Sue4
Thu Apr 25, 2013 7:37 pm
 
Forum: Cell Biology
Topic: Carnoy's fixation problem
Replies: 0
Views: 1903
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