Precipitation

... in a conical tube by centrifugation. Supernatant is the liquid phase atop of the pellet after centrifugation. Sometimes a pellet is formed after precipitation or other such event, if the precipitated molecule is sedimented to the bottom of the tube by gravity (the Earth is our centrifuge here). ...

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... aliquot was TCA precipitated to determine the amount of radioactivity incorporated into the DNA. The results were as follows: Counts before TCA precipitation = 374248 cpm Counts after TCA precipitation = 4905 cpm If the reaction was to be repeated, how many minutes would you allow the reaction ...

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... are pobably less concentrtated in the body, and there is indeen selection of ions both by the osteoblasts and the chemical reaction causing the Ca precipitation.

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... actually depends on what you'll do next and on the bugger (for western we put for 5 min in 95 and it's ok, right?). You could also try a different precipitation method.

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Dear, thnx for reply.actually as i tld i've precipitated protein by acetone precipitation. In this precipitation method we get pellet which is protein and after centrifugation v have to collect the pellet and dissolve it into resuspension buffer.my problem is ...

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