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Precipitation

Precipitation

  • the act or process of precipitating.

Origin: L. Praecipitatio


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TCA preipitation

Hi there, I have to do a cell wall extraction on bacteria soon and my protein of interest is cell wall anchored. My supervisor told me to do TCA precipitation (haven't done before) but I assume it's too isolate the proteins within the cell wall?? He told me I would have three components by the ...

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by biology_06er
Wed Feb 27, 2013 3:25 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 2494

Idea to determine order of assembly of spliceosome

Aha ok. Thank you very much, I just received a mail from my teacher and he actually suggested I do the precipitation, just as you did. From one thing to another though. What is the purpose of a bacteriophage promoter being ligated in to a vector along with your gene? Is it ...

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by nukeman
Wed May 30, 2012 6:14 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7817

DNA extraction forms very a viscous substance

... buffer (lyses buffer), a very viscous, mucus like substance results. If I carry on the protocol as recomended by Quiagen and add the AP2 buffer (precipitation buffer), centrifuge and after which I atempt to recover the supernatant, in several cases there is no supernatant to recovery. Just a ...

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by calobo
Tue Jan 10, 2012 5:21 pm
 
Forum: Molecular Biology
Topic: DNA extraction forms very a viscous substance
Replies: 1
Views: 1577

Re: I hate to do this.

... movement of resources within tropical forest ecosystem. The ecologist is monitoring the processes of evaporation, condensation, transpiration, and precipitation over a 180 day period. Which of the following cycles is the ecologist studying? water cycle. Correct. I hope this helps you at least a ...

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by Larsa
Tue Nov 15, 2011 11:07 am
 
Forum: General Discussion
Topic: I hate to do this.
Replies: 3
Views: 23640

I hate to do this.

... movement of resources within tropical forest ecosystem. The ecologist is monitoring the processes of evaporation, condensation, transpiration, and precipitation over a 180 day period. Which of the following cycles is the ecologist studying? water cycle.

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by biologyconfusesme
Mon Nov 14, 2011 7:39 pm
 
Forum: General Discussion
Topic: I hate to do this.
Replies: 3
Views: 23640
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