Dictionary » P » Precipitant

Precipitant

precipitant

(Science: chemistry) Any force or reagent which causes the formation of a precipitate.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Re: Porblems making DIG probe - please help

... matching the polymerase to the liberalized vector. The DIG mix contains everything and it’s the RNA mix. I know that polymerase buffers have a precipitant and I vortex and even heat a bit before use. Do you have any insight regarding incubation at 42 instead of 37? How is it best to purify ...

See entire post
by chaka8
Tue Jul 29, 2008 11:42 am
 
Forum: Molecular Biology
Topic: Porblems making DIG probe - please help
Replies: 5
Views: 4993

Folin-Wu Protein Precipitation method

The acid addition is because the precipitant works in acidic pH. For estimation Cl- and K+, as far as I know, there is no problem to use whether serum or plasma since the different between them is only the presence of coagulation factors ...

See entire post
by Dr.Stein
Tue Mar 13, 2007 3:24 am
 
Forum: Molecular Biology
Topic: Folin-Wu Protein Precipitation method
Replies: 4
Views: 6594

Folin-Wu Protein Precipitation method

... serum deproteinisation for a biochem subject, I am quite lost... just wondering why the acid (H2SO4) must be added to the serum before the anionic precipitant (10% sodium tungstate)? Also, for Cl- and K+ estimations, is it better to use serum or plasma? THANKS A LOT!

See entire post
by darkchoc
Tue Mar 13, 2007 1:39 am
 
Forum: Molecular Biology
Topic: Folin-Wu Protein Precipitation method
Replies: 4
Views: 6594


This page was last modified 21:16, 3 October 2005. This page has been accessed 1,328 times. 
What links here | Related changes | Permanent link