Precipitant

... matching the polymerase to the liberalized vector. The DIG mix contains everything and it’s the RNA mix. I know that polymerase buffers have a precipitant and I vortex and even heat a bit before use. Do you have any insight regarding incubation at 42 instead of 37? How is it best to purify ...

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The acid addition is because the precipitant works in acidic pH. For estimation Cl- and K+, as far as I know, there is no problem to use whether serum or plasma since the different between them is only the presence of coagulation factors ...

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... serum deproteinisation for a biochem subject, I am quite lost... just wondering why the acid (H2SO4) must be added to the serum before the anionic precipitant (10% sodium tungstate)? Also, for Cl- and K+ estimations, is it better to use serum or plasma? THANKS A LOT!

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