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Porin

Definition

noun, plural: porins

A transmembrane protein that assemble together with other porins to form a pore across membranes (i.e. plasma membrane, mitochondrial membrane, and nuclear envelope)


Supplement

Porins are proteins that assemble to form a complex that acts as channel where molecules can pass through. Examples of eukaryotic porins include the mitochondrial membrane porins that make up the voltage-dependent anion-selective channel and nucleoporins that form the nuclear pore complex.


Abbreviation / Acronym: por(e) + -in

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Porin-deficient mutant

Now suppose that the porin actually transports more than one substance - say, it can act on succinic acid as well as tetracycline. How might you figure out whether the porin is working or not? :)

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by AstraSequi
Wed Apr 11, 2012 2:37 am
 
Forum: Microbiology
Topic: Porin-deficient mutant
Replies: 4
Views: 2693

Re: Porin-deficient mutant

Bacteria can develop resistance to the antibiotic by mutating the gene that encodes the porin – the antibiotic is then excluded from passing through the outer membrane

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by Layd33foxx
Sun Apr 08, 2012 5:45 pm
 
Forum: Microbiology
Topic: Porin-deficient mutant
Replies: 4
Views: 2693

Re: Porin-deficient mutant

Porins are channels control the transport of substances in and out of the cell, imparting the membrane its property of semipermeabililty.

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by Layd33foxx
Sun Apr 08, 2012 5:33 pm
 
Forum: Microbiology
Topic: Porin-deficient mutant
Replies: 4
Views: 2693

Porin-deficient mutant

What is the function of a porin? Knowing that, what sort of experiment might you design to see whether or not the porin is present? A system where the cell dies if the porin is not present (that is, the cell can't execute the porin's function) ...

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by AstraSequi
Sat Apr 07, 2012 6:44 am
 
Forum: Microbiology
Topic: Porin-deficient mutant
Replies: 4
Views: 2693

Porin-deficient mutant

:?: Some bacteria become resistant to tetracycline because they don't make porins. Why can a porin-deficient mutant be detected by its inability to grow on a medium containing a single carbon source such as succinic acid?

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by Layd33foxx
Fri Apr 06, 2012 5:26 am
 
Forum: Microbiology
Topic: Porin-deficient mutant
Replies: 4
Views: 2693
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