Polymorphic

How different about DNA amplified band and polymorphic band? I would like to calcualte %of polymorphic band after PCR and electrophoresis, please explian. Thank you very much

See entire post

... and what is rare. Polymorphism means "many forms", whether molecular or morphological. So mutation creates polymorphism. The different polymorphic forms are variants, so "polymorphism" and "variant" are essentially synonymous. It is my opinion that much of the confusion ...

See entire post

What's a tandem repeat and what's a polymorphic region?

See entire post

... all positions. But if you PCR genomic DNA from a diploid organism and sequence the PCR product, you will get double peaks at all sites that are polymorphic. If your primers aren't specific for one gene of a gene family, you can amplify more than one gene and get multiple peaks for each site ...

See entire post

... a normal, programmed process that most eukaryotes undergo. Unequal crossing over and gene conversion are rare variants on the theme that lead to polymorphic states outside the recombinatorial norm. Including these events as mutational is quite valid. If you want to define mutation as any change ...

See entire post