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Polymerases

polymerase

(Science: enzyme) enzymes that catalyse the synthesis of nucleic acids on preexisting nucleic acid templates, assembling rNA from ribonucleotides or dNA from deoxyribonucleotides.


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A question regarding PCR

... is how many base pairs the amplified product will have, but it's not clear to me how to answer it. When the two DNA strand separate and have polymerases amplifying them, won't the initial products have different lengths since there is this little acatttg sequence before the first primer position? ...

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by x555
Sat Jan 18, 2014 11:05 am
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 467

Why is ATP rather than GTP, CTP or TTP the energy currency?

... soon as ATP was being used/produced a bit more than the others, a feedback loop set in, and it became dominant. Obviously, all 4 are used by DNA polymerases, and GTP is used by numerous receptor proteins. I'm not aware of much use for TTP or CTP, but GTP is prominent. So, as JackBean said: "(hint ...

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by BlahBlahBlah
Fri Jul 12, 2013 1:12 pm
 
Forum: Molecular Biology
Topic: Why is ATP rather than GTP, CTP or TTP the energy currency?
Replies: 5
Views: 3477

Help please with viruses?

... in its capsid? Does the capsid contain any enzymes? How does the virus enter the host cell? How is its genome replicated? Does it encode its own polymerases? Which ones? Where does it replicate its genome? Where is the virus assembled? How does it get out of the cell? How does it get to the kidneys? ...

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by leongs04
Tue Jul 24, 2012 4:13 am
 
Forum: Microbiology
Topic: Help please with viruses?
Replies: 2
Views: 2325

Re: Isolation during transcription

... If all you want to do is isolate some RNA polymerase, there are far easier ways to do it (and which don't involve viruses). In fact, some of these polymerases are sold commercially. then you need a technique to synthesis telomerase from the polymerase If you want to make telomerase in vitro , you ...

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by AstraSequi
Fri May 04, 2012 7:12 pm
 
Forum: Molecular Biology
Topic: Isolation during transcription
Replies: 2
Views: 1443

T7 Expression System

... the control of an E.Coli promoter into the cells, and induce it in the presence of labelled amino acids, and Rifampicin to inhibit endogenous RNA polymerases in the hope of translating the gene of interest, labelled, and easily purified. My question is - knowing that the T7 RNA polymerase can ...

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by TjCooper
Sat Dec 31, 2011 5:46 pm
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 2980
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