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Polymerases

polymerase

(Science: enzyme) enzymes that catalyse the synthesis of nucleic acids on preexisting nucleic acid templates, assembling rNA from ribonucleotides or dNA from deoxyribonucleotides.


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Help please with viruses?

... in its capsid? Does the capsid contain any enzymes? How does the virus enter the host cell? How is its genome replicated? Does it encode its own polymerases? Which ones? Where does it replicate its genome? Where is the virus assembled? How does it get out of the cell? How does it get to the kidneys? ...

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by leongs04
Tue Jul 24, 2012 4:13 am
 
Forum: Microbiology
Topic: Help please with viruses?
Replies: 2
Views: 1567

Re: Isolation during transcription

... If all you want to do is isolate some RNA polymerase, there are far easier ways to do it (and which don't involve viruses). In fact, some of these polymerases are sold commercially. then you need a technique to synthesis telomerase from the polymerase If you want to make telomerase in vitro , you ...

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by AstraSequi
Fri May 04, 2012 7:12 pm
 
Forum: Molecular Biology
Topic: Isolation during transcription
Replies: 2
Views: 997

T7 Expression System

... the control of an E.Coli promoter into the cells, and induce it in the presence of labelled amino acids, and Rifampicin to inhibit endogenous RNA polymerases in the hope of translating the gene of interest, labelled, and easily purified. My question is - knowing that the T7 RNA polymerase can ...

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by TjCooper
Sat Dec 31, 2011 5:46 pm
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 2120

Re: Mismatch DNA repair

Increased efficiency? The processes of strand elongation and primer replacement are spatially/temporally separated, it's faster if 2 polymerases do it at once. Another way of looking at it... proofreading is such an important ability that if one polymerase is defective, the other one still ...

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by TheWalkingGlitch
Wed Nov 09, 2011 1:21 am
 
Forum: Molecular Biology
Topic: Mismatch DNA repair
Replies: 4
Views: 2370

the RNA synthesized on one of the DNA strands is ..........?

Yes, it is all of them. All RNAs are synthesized by RNA polymerases, and all RNA polymerases use one strand of DNA as the template strand to synthesize single-stranded RNA.

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by aptitude
Tue Nov 08, 2011 6:38 am
 
Forum: Cell Biology
Topic: the RNA synthesized on one of the DNA strands is ..........?
Replies: 4
Views: 1571
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