Dictionary » P » Polymerase

Polymerase

polymerase

(Science: enzyme) enzymes that catalyse the synthesis of nucleic acids on preexisting nucleic acid templates, assembling rNA from ribonucleotides or dNA from deoxyribonucleotides. An enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA.An [[enzyme promoting the joining together of [[nucleic acids with one another.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Annealing Temparature

... at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, so 1 min can be enough for Phusion, but 4 min will be required for Pfu.

See entire post
by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 1333

Annealing Temparature

... in the beginning is only 1 min or is it a typo? Why do you have such long denaturation during cycling? Why do you have such long annealing? What polymerase do you use? What is the GC content of your GOI? How many cycles did you have? What is the template? What concentration did you use?

See entire post
by JackBean
Tue Feb 18, 2014 12:40 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 1333

A question regarding PCR

probably, your microsatellite is part of the chromosome, right? So there will be much more than 7 bp and it will be on both sides. Yes, the polymerase will amplify the chain until the conditions change (i.e. end of amplification step) or until it falls off (which may happen with very long amplicons, ...

See entire post
by JackBean
Sat Jan 18, 2014 2:36 pm
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 1253

Taq Buffer ?

why Taq DNA Polymerase Buffers containing Gelatin ?

See entire post
by Helics
Sat Jan 18, 2014 9:36 am
 
Forum: Molecular Biology
Topic: Taq Buffer ?
Replies: 1
Views: 533

microsatellite instability

... When PCR amplifying microsatellite markers for subsequent fragment analysis by microcapillary elfo - is it important to use an ultra-high fidelity polymerase, or is it not important?

See entire post
by kk
Tue Oct 08, 2013 9:47 am
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2175
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 11,451 times. 
What links here | Related changes | Permanent link