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Dictionary » P » Polymerase

Polymerase

polymerase

(Science: enzyme) enzymes that catalyse the synthesis of nucleic acids on preexisting nucleic acid templates, assembling rNA from ribonucleotides or dNA from deoxyribonucleotides. An enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA.An [[enzyme promoting the joining together of [[nucleic acids with one another.


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Re: PCR & Agarose gel electrophoresis

Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the explanation to that. ...

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by apolll
Sun Aug 23, 2009 8:40 pm
 
Forum: Molecular Biology
Topic: PCR & Agarose gel electrophoresis
Replies: 3
Views: 203

influence of temperature on biomolecules

... and shape) 3) protein-protein interactions (again, affected also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors Thanks a lot

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by biobynight
Thu Aug 20, 2009 8:37 pm
 
Forum: Molecular Biology
Topic: influence of temperature on biomolecules
Replies: 0
Views: 82

Cellular processes affected by temperature..

... and shape) 3) protein-protein interactions (again, affected also by shape/bi-stability) 4) transcription rate and errors (TF to DNA binding rate, polymerase binding rate, chromatin effects) 5) translation rate and errors

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by biobynight
Thu Aug 20, 2009 8:34 pm
 
Forum: Cell Biology
Topic: LIfe processes affected by temperature..
Replies: 3
Views: 623

Paramutation

... Stability of the chromatin states associated with paramutation and transposon silencing requires the mop1 gene, which encodes an RNA-dependent RNA polymerase." This polymerase is required to maintain a threshold level of the repeat RNA, which causes the paramutation. Exactly how the RNA does ...

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by koan
Thu Aug 20, 2009 8:10 pm
 
Forum: Molecular Biology
Topic: Paramutation
Replies: 5
Views: 149

Re: Ligation and expression trouble

... is mainly that bases can be lost at the ends, or that it can ligate and produce a molecule that is missing a few bases, which get filled in by the polymerase of the bacterium after you transform your ligation mix. Oftentimes i'll get a whole bunch of re-ligated vector when I pick my colonies and ...

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by MrMistery
Thu Jul 09, 2009 12:41 am
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 433
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