Dictionary » P » Polymerase

Polymerase

polymerase

(Science: enzyme) enzymes that catalyse the synthesis of nucleic acids on preexisting nucleic acid templates, assembling rNA from ribonucleotides or dNA from deoxyribonucleotides. An enzyme that catalyzes the formation of new DNA and RNA from an existing strand of DNA or RNA.An [[enzyme promoting the joining together of [[nucleic acids with one another.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Taqman probe design using FISH probe (need help)

... temperature such that it will remain hybridized at PCR extension temperature (but not so high as a fish probe) and will be hydrolysed by Taq DNA polymerase at every replication, thus releasing the fluorescence (quenched in intact probe) that will be measured to quantify the starting level of ...

See entire post
by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 582

Re: Purpose of a primase/polymerase localized to the cytosol?

Hi, I have some naïve thoughts about this situation: If it is an RNA pol, it may be helping maintain/regulate RNA levels in cytosol by e.g. recharging decaying RNA levels in cytosols for extended translation. If it is a DNA pol, it might be a long surviving vestigial remnant of bacterial ancestry. I...

See entire post
by Hrushikesh
Fri Dec 12, 2014 8:40 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 2
Views: 1602

Purpose of a primase/polymerase localized to the cytosol?

Hi, I recently read a paper, "PrimPol, an Archaic Primase/Polymerase Operating in Human Cells" by Garcia-Gomez et al. This polymerase was largely localized to the cytosol - I'm wondering if anyone has ideas as to why that would be the case or ...

See entire post
by rhardison
Fri Oct 03, 2014 8:51 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 2
Views: 1602

Re: Any ideas for vaccine development or anti-viral drugs???

... oligomers (PMOs). A combination of EBOV-specific PMOs targeting sequences of viral mRNAs for the viral proteins (VPs) VP24, VP35, and RNA polymerase L protected rodents in both pre- and post-exposure therapeutic regimens. In a prophylactic proof-of-principal trial, the PMOs also protected ...

See entire post
by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12730

Annealing Temparature

... at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, so 1 min can be enough for Phusion, but 4 min will be required for Pfu.

See entire post
by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 2615
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 13,816 times. 
What links here | Related changes | Permanent link