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Polyclonal

polyclonal

Derived from different types of cells.


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Animal-Derived Laboratory Reagents

... I right in thinking this? • In vivo (ascites) monoclonal antibodies: Does an animal have to die every time I place a new order for an antibody? • Polyclonal antibodies: Again, does an animal have to die every time I place a new order for an antibody? Sheep’s blood agar: Unlike the bovine products, ...

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by southtowns18
Thu Dec 06, 2012 6:13 pm
 
Forum: General Discussion
Topic: Animal-Derived Laboratory Reagents
Replies: 0
Views: 1171

Re: Western Blot TroubleShooting

... in the transblot solution for 15 min before I start the transfer. The transfer is done in the cold. I block in 5% milk + TBST. I have tried two polyclonal rabbit primary antibodies against my protein of interest. I use an antirabbit-HRP secondary antibody.

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by mutant11
Mon Nov 21, 2011 3:11 am
 
Forum: Molecular Biology
Topic: Western Blot TroubleShooting
Replies: 7
Views: 3352

simple epitope

you should be able to to raise antibodies only against your epitope (understand - use only that epitope for imunization), or raise polyclonal antibodies and lok, which one will best bind your epitope

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by JackBean
Tue Jan 18, 2011 11:06 am
 
Forum: General Discussion
Topic: simple epitope
Replies: 5
Views: 1994

antibody staining

... the context. In immunology, when talking about poly- vs. monoclonal antibodies, the specificity of the antibody can be the same antigen, just the polyclonal antibody recognizes many different epitopes and monoclonal just one. Polyclonal antibodies against a single antigen can be produced in animals ...

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by biohazard
Tue Nov 03, 2009 1:27 pm
 
Forum: Cell Biology
Topic: antibody staining
Replies: 2
Views: 1707

antibody staining

Sure, polyclonal antibody can recognise like everything...

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by JackBean
Tue Nov 03, 2009 11:13 am
 
Forum: Cell Biology
Topic: antibody staining
Replies: 2
Views: 1707
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