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Dictionary » P » Polyacrylamide gel electrophoresis

Polyacrylamide gel electrophoresis

polyacrylamide gel electrophoresis

analytical and separative technique in which molecules, particularly proteins, are separated by their different electrophoretic mobilities in a hydrated gel.

The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. Commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (sDS).

sDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight.

See: sDS pAGE.


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maybe 2-D polyacrylamide gel electrophoresis helpful for you, you can seperate, identify, and measure ...

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... Dr. U. K. Laemmli developed a system of gel electrophoresis through a plastic medium (polyacrylamide) that easily allowed for proteins to be separated ...

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... Gel+Materials%23rpp%3D15&fromSearch=1&searchKey=agarose&highlightProductsItemsFlag=Y ... but using Low melting agarose for regular electrophoresis is a waste of money. The OP has ... are that small, it would make sense to run a polyacrylamide gel any way.

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Pulse-field gel electrophoresis is good for large DNA fragments. yeah, that's ... @Cmgross: before going for agarose gel elctrophoresis or polyacrylamide gel electrophoresis, go for PCR amlification for ...

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by JackBean
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