Polyacrylamide

... fragment, I cannot see why they are using such a high agarose concentration. And if the differences are that small, it would make sense to run a polyacrylamide gel any way.

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... mentioned that he was unsure whether a 100kb size genome would run properly. Support you. @Cmgross: before going for agarose gel elctrophoresis or polyacrylamide gel electrophoresis, go for PCR amlification for your desired quantity. Why should he run PCR? How will that help?

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... mentioned that he was unsure whether a 100kb size genome would run properly. Support you. @Cmgross: before going for agarose gel elctrophoresis or polyacrylamide gel electrophoresis, go for PCR amlification for your desired quantity.

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... of how many proteins there are in the mixture? Me: I know that you have to use agarose gels on native proteins instead of something like a polyacrylamide matrix with SDS or 2-ME which are used to move denatured proteins electrophoretically. Agarose gels have pores large enough to allow ...

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Okay, i ran HRP with polyacrylamide gel electrophoresis, the buffer that i used to store the HRP is SOdium phosphate monohydrate and it worked out alright. I loaded the wells with beta-mercaptoethanol and all the proteins had sodium ...

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