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Polyacrylamide

polyacrylamide

A branched polymer of acrylamide (H2C==CHCONH2) that is used in gel electrophoresis; e.g., R-CH2-CH(CONH2)-CH(CONHRCH(CONHR')-R'.


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protein fractionation

maybe 2-D polyacrylamide gel electrophoresis helpful for you, you can seperate, identify, and measure whole proteins in yur cell sample

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by drfuture
Mon May 20, 2013 3:34 pm
 
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 2094

Western Blot

... related to their molecular size. Using this feature, Dr. U. K. Laemmli developed a system of gel electrophoresis through a plastic medium (polyacrylamide) that easily allowed for proteins to be separated by an electric current strictly according to their overall mass. (1)

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by MarkHolland
Sat Mar 30, 2013 3:22 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 2609

Re:

... fragment, I cannot see why they are using such a high agarose concentration. And if the differences are that small, it would make sense to run a polyacrylamide gel any way.

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by canalon
Tue Nov 08, 2011 1:55 am
 
Forum: Genetics
Topic: DNA Gel Electrophoresis Issues - Bad Ladder Separation
Replies: 4
Views: 6894

Re:

... mentioned that he was unsure whether a 100kb size genome would run properly. Support you. @Cmgross: before going for agarose gel elctrophoresis or polyacrylamide gel electrophoresis, go for PCR amlification for your desired quantity. Why should he run PCR? How will that help?

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by JackBean
Thu Oct 20, 2011 8:18 am
 
Forum: Molecular Biology
Topic: distinguishing circular and linear DNA
Replies: 9
Views: 10646

Re: distinguishing circular and linear DNA

... mentioned that he was unsure whether a 100kb size genome would run properly. Support you. @Cmgross: before going for agarose gel elctrophoresis or polyacrylamide gel electrophoresis, go for PCR amlification for your desired quantity.

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by greatmicrobiologist
Mon Sep 12, 2011 12:22 pm
 
Forum: Molecular Biology
Topic: distinguishing circular and linear DNA
Replies: 9
Views: 10646
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