Pol gene

We use RNAi and Lentiviral transgenesis in our lab to generate transgenic mice. We use luceferase assay to validate the shRNA sequences. The problem is the results don't seem to traslate well into in vitro systems. I analysed 8 sequences ...

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Researchers have figured out a potential way to prevent mitochondrial DNA related diseases by trading out healthy mitochondria for the mutated mitochondria. Apparently there are no side effects. Read more

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All genes are necessary and required.. that's the general rule of genomics. It's mutations in genes that affect the "good" or "bad" effects of the gene in particular. Considering the pathway the p21 gene ...

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... us unwanted banding in our gels and it turned out that our primers were fine. Maybe you could try ordering a primer for a different section of the gene? We usually order 2-3 different primers for any given gene we're testing for.. after all, it is pretty inexpensive.

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Hi I designed a specific primers and blast it to amplify my gene. this primer amplifies a gDNA of the gene. I used dreamtaq kit and its protocol but unfortunately the expected product (2054 bp) was not there and what I got is several bands below and above ...

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