Dictionary » P » Pol gene

Pol gene

pol gene

(Science: molecular biology) genes coding for dNA polymerases of which there are three in e. Coli, polA, polB and polC coding for polymerases I, II and III respectively.

Pol genes in oncogenic retroviruses code for reverse transcriptase.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Idea to determine order of assembly of spliceosome

... a bacteriophage promoter being ligated in to a vector along with your gene? Is it just to ensure that you get strong expression if you use some kind of phage RNA-pol?

See entire post
by nukeman
Wed May 30, 2012 6:14 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7555

Idea to determine order of assembly of spliceosome

... vitro splicing assay with a couple of extra steps. 1. Ligate a traget gene to a vector with some kind of bacteriophage RNA-pol promotor 2. In vitro transcription with radioactive nucleotides 3. Extraction of pre-mRNA ...

See entire post
by nukeman
Mon May 28, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7555

what is the difference between promoter and enhancer

Promotor is part of DNA adjacent to the gene (just few nucleotides ahead of transcripted sequence), which is crucial for the transcription. No promotor = no transcription. It's the place where the RNA Pol binds. Enhancer can be adjacent, but also far away from the transcription start ...

See entire post
by JackBean
Wed Mar 14, 2012 9:59 am
 
Forum: Molecular Biology
Topic: what is the difference between promoter and enhancer
Replies: 3
Views: 5115

Re: no repeat sequences in exons of genes?

... so retrotransposed would lead to the creation of an extra locus for the gene of interest.....remember its very unlikely to hit a piece of protein ... code for proteins it might code for viral proteins, or gobbledeegook polypeptides. In fact you should have been told that these transposons often ...

See entire post
by merv
Mon Oct 03, 2011 1:37 am
 
Forum: Genetics
Topic: no repeat sequences in exons of genes?
Replies: 5
Views: 10417

LIC insertion for dual protein expression

Dear All, I currently have a gene coding for a two domain protein cloned into an expression vector (pET28b). I would ... its 3' end. I will obtain then a linearized vector that, after treatment with T4 Pol+dATP I can anneal with a pETDuet LIC adaptor that will provide the T7 promoter, ...

See entire post
by cpmf752004
Mon May 23, 2011 7:21 pm
 
Forum: Molecular Biology
Topic: LIC insertion for dual protein expression
Replies: 0
Views: 857
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 2,834 times. 
What links here | Related changes | Permanent link