Pol gene

... a bacteriophage promoter being ligated in to a vector along with your gene? Is it just to ensure that you get strong expression if you use some kind of phage RNA-pol?

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... vitro splicing assay with a couple of extra steps. 1. Ligate a traget gene to a vector with some kind of bacteriophage RNA-pol promotor 2. In vitro transcription with radioactive nucleotides 3. Extraction of pre-mRNA ...

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Promotor is part of DNA adjacent to the gene (just few nucleotides ahead of transcripted sequence), which is crucial for the transcription. No promotor = no transcription. It's the place where the RNA Pol binds. Enhancer can be adjacent, but also far away from the transcription start ...

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... so retrotransposed would lead to the creation of an extra locus for the gene of interest.....remember its very unlikely to hit a piece of protein ... code for proteins it might code for viral proteins, or gobbledeegook polypeptides. In fact you should have been told that these transposons often ...

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Dear All, I currently have a gene coding for a two domain protein cloned into an expression vector (pET28b). I would ... its 3' end. I will obtain then a linearized vector that, after treatment with T4 Pol+dATP I can anneal with a pETDuet LIC adaptor that will provide the T7 promoter, ...

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