Pnp

... 30±18 Fetuin 445±35 Mucin 377±35 Ganglioside GM1 30±12 Ganglioside GD1 25±10 The enzyme was also strongly inhibited by Neu5Ac, 2en and weakly by pNPO. Evaluation of the inhibition against Mu-Neu5Ac as substrate revealed competitive patterns (Fig. 3) for both Neu5Ac2en and pNP-oxamic acid with ...

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... added 0,1 mL 0,25 mM p-nitrophenol in 0.05 M glycine buffer, pH = 9.5 to 1,9 mL glycine buffer ? if so, then the dilution factor is 20 and thus [PNP] = 0.0125 mM --> in a volume of 2 mL, amount of mmol PNP = 0.0125 x 10^-3 * 2 = 2.5 x 10^-5 mmol --> thus amount nmol PNP = 2.5 x 10^-5 * ...

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