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Plating

plating

1. The art or process of covering anything with a plate or plates, or with metal, particularly of overlaying a base or dull metal with a thin plate of precious or bright metal, as by mechanical means or by electromagnetic deposition.

2. A thin coating of metal laid upon another metal.

3. A coating or defensive armor of metal (usually steel) plates.


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How to maintain sterility while preparing petri dishes

... Properly heated agar poured on sterile plastic dishes should give you sterile agar plates in most cases even if done without autoclaves and hoods. Plating the bacteria shouldn't be a problem: even if you open the lids for as long as several minutes, you are likely to get no contaminating bacteria ...

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by biohazard
Mon Jun 24, 2013 8:56 am
 
Forum: Microbiology
Topic: How to maintain sterility while preparing petri dishes
Replies: 8
Views: 4285

How to maintain sterility while preparing petri dishes

... they also stay sterile for several weeks in the refrigerator. Sterile plastic loops (or alternatively Bunsen-sterilized metal ones) are good for plating the bacteria. This we have done simply on the desktop without any real contamination issues. Actually by far the most common contamination from ...

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by biohazard
Mon Jun 24, 2013 7:12 am
 
Forum: Microbiology
Topic: How to maintain sterility while preparing petri dishes
Replies: 8
Views: 4285

Amp selection with no recovery

For years, I have been doing E. coli transformation by heat shocking cells and then plating immediately on amp plates with no recovery. I was taught this trick years ago and was told that the reason it worked had to do with the fact that amp affects cell wall biosynthesis ...

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by daknecht
Sat Dec 08, 2012 4:54 pm
 
Forum: Molecular Biology
Topic: Amp selection with no recovery
Replies: 1
Views: 1367

Re: Colony growth

... anything now. We have done away with our mixed culture stock broths and and various agar plates. We have only covered staining and various plating methods thus far or I would have used more test to be sure.... Out of our list of unknowns I have 4 that cold grow on the Mac, so I will just ...

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by sci2012
Sun Mar 11, 2012 12:47 am
 
Forum: Microbiology
Topic: Colony growth
Replies: 2
Views: 2242

Re: HELP! Why do I keep getting a mixed culture in my gramstain?

my plate count agar (Pour plating from a potable water) got green pigmentation that didnt occur usually.maybe it was pseudomonas,. i think You know what you could be right. I actually got mine to be pure culture after redoing the gram ...

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by SweetSarah
Sun Feb 26, 2012 5:55 am
 
Forum: Microbiology
Topic: HELP! Why do I keep getting a mixed culture in my gramstain?
Replies: 11
Views: 7404
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