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Plasmid

Definition

noun, plural: plasmids

A linear or circular double-stranded DNA that is capable of replicating independently of the chromosomal DNA.


Supplement

Certain plasmids are able to insert themselves into the chromosomes particularly in regions where there is a common sequence of nucleotides. Hence, they are used in recombinant DNA technology and research as means for transferring genes between cells or as cloning vectors.

Plasmids are important in certain bacteria since plasmids code for proteins, especially enzymes, which can confer resistance to antibiotics. Plasmids are ubiquitous in prokaryotes but they have also been found in a number of eukaryotes, e.g. Saccharomyces cerevisiae, which may contain a 2-micrometre-ring of plasmid.


Word origin: The term plasmid was first introduced by the American molecular biologist Joshua Lederberg in 1952.
Synonym: extrachromosomal element.
See also: DNA, chromosome.



User Contributions:

  • Plasmid (PLAZ mid) - a genetic structure that can replicate independently of the main chromosome(s) of a cell; usually, a circular DNA molecule in bacteria ( prokaryotes).

Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


5' LTR sequece

Hi, I want to check the 5' LTR sequence of my plasmid. I will like to confirm that it is a hybrid 5' LTR and not a wild type HIV-1 5' LTR. Can someone pls guide me on how I can evaluate this? Best, John

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by aguy
Mon Jul 07, 2014 10:02 pm
 
Forum: Molecular Biology
Topic: 5' LTR sequece
Replies: 0
Views: 162

Please guide me about these restriction enzymes

you must select enzyme that cut your plasmid in one (or two) determined sequense and also not your target gene.

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by hematologym
Mon Mar 17, 2014 11:17 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 953

Help: Confused with primers in a PCR of a vector?

... a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried out a PCR reaction of the pBS-gene (our clone). This PCR reaction is something I do not really understand. We used the pBS-gene ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 1896

Please guide me about these restriction enzymes

I guess that depends rather on your plasmid https://www.neb.com/products/r0189-noti https://www.neb.com/products/r0131-nhei

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by JackBean
Tue Mar 11, 2014 2:20 pm
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 953

Please guide me about these restriction enzymes

For linirization of plasmid yeast(cervizea)why NotI is used instead of NHEI? please compare and describe them

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by 2angel
Tue Mar 11, 2014 7:24 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 953
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