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Plasmid vector



Plasmid manipulated by genetic engineering to serve as a tool to produce the desired products in the target cell through a biomolecular process such by the expression of gene of interest.


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Help: Confused with primers in a PCR of a vector?

... familiar with what is going on. We recently ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried out a PCR reaction of the pBS-gene (our clone). This ...

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by keetner
Sat Mar 15, 2014 3:21 am
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2683


... from what I can say: Your location doesn't really matter for ordering plasmids, you will probably buy it from some big company anyway. Just find the best vector for you (including selection). GFP is IMHO suitable for single cell selection (or ...

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by JackBean
Thu Sep 05, 2013 11:41 am
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1808


... try to ligate annealed oligos into double digested (different enzymes) plasmid. The oligos I annealed were not modified with phosphates. Do I need ... it is not necessary to dephosphorylate my double-digested open vector as long as it was cut with two different enzymes. Thus, the 5'-end ...

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by kk
Tue Feb 12, 2013 2:48 pm
Forum: Molecular Biology
Replies: 9
Views: 5573

Re: Markerless mutants using suicide vector

... issue (and difference between the two) are not so obvious for us. Our vector is a circular plasmid. Can you elaborate on what you mean by two linear pieces that are homologous to each ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 2749

Cloning/Transformation question

... way to make negative control is to set up a ligation of your digested vector with water (or TE buffer/elution buffer you are using) in place of ... not work anymore. The other suggestion - transformation using wrong plasmid (another antibiotic resistance) - is also wrong and has no value.

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by Cat
Sat Dec 01, 2012 12:50 am
Forum: Molecular Biology
Topic: Cloning/Transformation question
Replies: 4
Views: 2426
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